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ZFIN ID: ZDB-PUB-120807-4
Visualization and exploration of Tcf/Lef function using a highly responsive Wnt/beta-catenin signaling-reporter transgenic zebrafish
Shimizu, N., Kawakami, K., and Ishitani, T.
Date: 2012
Source: Developmental Biology 370(1): 71-85 (Journal)
Registered Authors: Ishitani, Tohru, Kawakami, Koichi, Shimizu, Nobuyuki
Keywords: Tcf/Lef, Wnt/β-catenin signaling, zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/embryology*
  • Blotting, Southern
  • Cell Culture Techniques
  • DNA Primers/genetics
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology*
  • Genes, Reporter/genetics
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • HEK293 Cells
  • Humans
  • In Situ Hybridization
  • Lateral Line System/embryology*
  • Plasmids/genetics
  • Polymerase Chain Reaction
  • Signal Transduction/genetics
  • Signal Transduction/physiology*
  • TCF Transcription Factors/genetics
  • TCF Transcription Factors/physiology*
  • Wnt Proteins/genetics
  • Wnt Proteins/metabolism
  • Zebrafish/embryology*
  • beta Catenin/genetics
  • beta Catenin/metabolism
PubMed: 22842099 Full text @ Dev. Biol.
FIGURES
ABSTRACT

Evolutionarily conserved Tcf/Lef transcription factors (Lef1, Tcf7, Tcf7l1, and Tcf7l2) mediate gene expression regulated by Wnt/β-catenin signaling, which has multiple roles in early embryogenesis, organogenesis, adult tissue homeostasis, and tissue regeneration. However, the spatiotemporal dynamics of Tcf/Lef activity during these events remain poorly understood. We generated stable transgenic zebrafish lines carrying a new Wnt/β-catenin signaling reporter, Tcf/Lef-miniP:dGFP. The reporter revealed the transcriptional activities of four Tcf/Lef members controlled by Wnt/β-catenin signaling, which were expressed in known Wnt/β-catenin signaling-active sites during embryogenesis, organ development and growth, and tissue regeneration. We used the transgenic lines to demonstrate the contribution of Tcf/Lef-mediated Wnt/β-catenin signaling to the development of the anterior lateral line, dorsal and secondary posterior lateral lines, and gill filaments. Thus, these reporter lines are highly useful tools for studying Tcf/Lef-mediated Wnt/β-catenin signaling-dependent processes.

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