PUBLICATION
Techniques for the in vivo assessment of cardio-renal function in zebrafish (Danio rerio) larvae
- Authors
- Rider, S.A., Tucker, C.S., Del-Pozo, J., Rose, K.N., MacRae, C.A., Bailey, M.A., and Mullins, J.J.
- ID
- ZDB-PUB-120216-8
- Date
- 2012
- Source
- The Journal of physiology 590(8): 1803-1809 (Journal)
- Registered Authors
- MacRae, Calum A.
- Keywords
- renal function, zebrafish, inulin
- MeSH Terms
-
- Animals
- Cardiovascular Physiological Phenomena
- Heart/physiology*
- Inulin/metabolism
- Kidney Function Tests
- Kidney Glomerulus/physiology*
- Larva
- Models, Animal*
- Urinary Tract Physiological Phenomena
- Zebrafish/physiology*
- PubMed
- 22331420 Full text @ J. Physiol.
Citation
Rider, S.A., Tucker, C.S., Del-Pozo, J., Rose, K.N., MacRae, C.A., Bailey, M.A., and Mullins, J.J. (2012) Techniques for the in vivo assessment of cardio-renal function in zebrafish (Danio rerio) larvae. The Journal of physiology. 590(8):1803-1809.
Abstract
Zebrafish, a well-established vertebrate model, offer unique advantages for assessing renal function and physiology. Assays
determining renal glomerular function based on cardiovascular erythrocyte flow and reduction of injected FITC-inulin were
developed; each validated using the nephrotoxin gentamicin.
Bland-Atlman analysis showed a strong association between measurements of the rate of inulin excretion and that of fluorescent
reduction from the arterial vasculature. Reduced renal clearance of inulin, resulting from gentamicin or NaCl loading, was
concurrent with reduced erythrocyte velocity, and yolk sac and pericardium oedema. These techniques, assessing pronephric
function, highlight the potential for in-vivo physiological study in this genetically tractable model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping