PUBLICATION
Quantifying cardiac functions in embryonic and adult zebrafish
- Authors
- Hoage, T., Ding, Y., and Xu, X.
- ID
- ZDB-PUB-120111-21
- Date
- 2012
- Source
- Methods in molecular biology (Clifton, N.J.) 843: 11-20 (Chapter)
- Registered Authors
- Ding, Yonghe, Hoage, Tiffany R., Xu, Xiaolei
- Keywords
- zebrafish, physiology, shortening fraction, heart rate, flow rate
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology*
- Erythrocytes
- Heart/embryology*
- Heart/physiology*
- Heart Rate
- Heart Ventricles/anatomy & histology
- Hemodynamics
- Molecular Imaging
- Organ Size
- Time Factors
- Ventricular Function
- Zebrafish/embryology*
- PubMed
- 22222517 Full text @ Meth. Mol. Biol.
Citation
Hoage, T., Ding, Y., and Xu, X. (2012) Quantifying cardiac functions in embryonic and adult zebrafish. Methods in molecular biology (Clifton, N.J.). 843:11-20.
Abstract
Zebrafish embryos have been extensively used to study heart development and cardiac function, mainly due to the unique embryology and genetics of this model organism. Since most human heart disease occurs during adulthood, adult zebrafish models of heart disease are being created to dissect mechanisms of the disease and discover novel therapies. However, due to its small heart size, the use of cardiac functional assays in the adult zebrafish has been limited. To address this bottleneck, the transparent fish line casper;Tg(cmlc2:nuDsRed) that has a red fluorescent heart can be used to document beating hearts in vivo and to quantify cardiac functions in adult zebrafish. Here, we describe our methods for quantifying shortening fraction and heart rate in embryonic zebrafish, as well as in the juvenile and adult casper;Tg(cmlc2:nuDsRed) fish. In addition, we describe the red blood cell flow rate assay that can be used to reflect cardiac function indirectly in zebrafish at any stage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping