ZFIN ID: ZDB-PUB-111122-11
Molecular characterization and expression analysis of two new C-reactive protein genes from common carp (Cyprinus carpio)
Falco, A., Cartwright, J.R., Wiegertjes, G.F., and Hoole, D.
Date: 2012
Source: Developmental and comparative immunology   37(1): 127-138 (Journal)
Registered Authors:
Keywords: C-Reactive protein, CRP, immune system, Cyprinus carpio, carp, fish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • C-Reactive Protein/genetics*
  • C-Reactive Protein/metabolism
  • Carps
  • Conserved Sequence
  • Fish Proteins/genetics*
  • Fish Proteins/metabolism
  • Gene Components
  • Gene Expression*
  • Gene Order
  • Molecular Sequence Data
  • Organ Specificity
  • Phylogeny
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Synteny
  • Zebrafish/genetics
PubMed: 22079493 Full text @ Dev. Comp. Immunol.
C-Reactive protein (CRP) plays an important role in the acute phase response. Transcripts encoding two new CRP-like molecules (ccCRP1 and ccCRP2) from European common carp have been characterized which has enabled seven CRP-like genes to be identified in zebrafish. 79.3% (ccCRP1) and 74.5% (ccCRP2) identity to CRP from East-Asian common carp occurs and fish CRP genes form a distinct clade. ccCRP2 gene organization comprises four exons and three introns, in contrast to the two exons/one intron organization of mammalian CRP genes. Gene expression assays showed both ccCRP-like molecules are constitutively expressed in liver, skin, gill, gut, muscle, kidney, spleen and blood. Protein levels of ccCRP in serum and spleen were significantly different from other organs analyzed, and levels were greatest in the liver. It is proposed that the two carp CRP genes defined differ in their expression profiles which may suggest differences in their biological activities.