PUBLICATION

Expression characterization and the promoter activity analysis of zebrafish hdac4

Authors
Zhu, K., Wang, H., Gul, Y., Zhao, Y., Wang, W., Liu, S., and Wang, M.
ID
ZDB-PUB-110927-2
Date
2012
Source
Fish physiology and biochemistry   38(2): 585-593 (Journal)
Registered Authors
Keywords
hdac4, zebrafish, TSA, expression, promoter activity
MeSH Terms
  • Animals
  • Base Sequence
  • Cricetinae
  • Embryonic Development/drug effects
  • Fish Proteins/antagonists & inhibitors
  • Fish Proteins/metabolism*
  • Histone Deacetylase Inhibitors/pharmacology
  • Histone Deacetylases/metabolism*
  • Hydroxamic Acids/pharmacology
  • MEF2 Transcription Factors
  • Mice
  • Molecular Sequence Data
  • Myogenic Regulatory Factors/antagonists & inhibitors
  • Myogenic Regulatory Factors/metabolism*
  • NIH 3T3 Cells
  • Promoter Regions, Genetic*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism*
PubMed
21773810 Full text @ Fish Physiol. Biochem.
Abstract

Histone deacetylase 4 (HDAC4) is an important modifier enzyme for chromatin remodeling and plays an essential role in regulating gene expression. Spatio-temporal expression spectrum revealed that zebrafish hdac4 mRNA, ubiquitously distributed in various tissues, were significantly higher at 36 hpf (hours post-fertilization) and 6 dpf (days post-fertilization) than other periods. Trichostatin A (TSA) inhibited the development of zebrafish embryos and transcription of hdac4 and mef2a (myocyte enhancer factor-2A). Moreover, five vectors containing different promoter regions of hdac4 were constructed in order to analyze promoter activity. The vector containing the region from -125 to +160 exhibited maximum luciferase activity that was approximately 30.3-fold and 58.9-fold higher than the control in two kinds of cells, respectively. By comparing the luciferase activities between the region from -302 to +30 and -698 to +30, it was suggested that the region between -698 and -302 might contain mild negative regulatory elements.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping