PUBLICATION

Engineering zinc finger nucleases for targeted mutagenesis of zebrafish

Authors
Sander, J.D., Yeh, J.R., Peterson, R.T., and Joung, J.K.
ID
ZDB-PUB-110921-5
Date
2011
Source
Methods in cell biology   104: 51-58 (Chapter)
Registered Authors
Peterson, Randall, Yeh, Jing-Ruey (Joanna)
Keywords
protein engineering, reverse genetics, targeted mutagenesis, zinc finger nucleases
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cloning, Molecular
  • Decision Trees
  • Deoxyribonucleases, Type II Site-Specific/genetics*
  • Genetic Vectors
  • Mutagenesis, Site-Directed/methods
  • Protein Engineering/methods*
  • Reverse Genetics/methods*
  • Zebrafish/genetics*
  • Zinc Fingers
PubMed
21924156 Full text @ Meth. Cell. Biol.
Abstract
Zinc finger nucleases provide an important platform for performing reverse genetics in zebrafish. Here we review various methods and resources that have been used to create customized zinc finger nucleases for use in zebrafish. We also provide a framework for choosing among the various publicly available platforms available to engineer ZFNs.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping