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ZFIN ID: ZDB-PUB-110921-5
Engineering zinc finger nucleases for targeted mutagenesis of zebrafish
Sander, J.D., Yeh, J.R., Peterson, R.T., and Joung, J.K.
Date: 2011
Source: Methods in cell biology   104: 51-58 (Chapter)
Registered Authors: Peterson, Randall, Yeh, Jing-Ruey (Joanna)
Keywords: protein engineering, reverse genetics, targeted mutagenesis, zinc finger nucleases
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cloning, Molecular
  • Decision Trees
  • Deoxyribonucleases, Type II Site-Specific/genetics*
  • Genetic Vectors
  • Mutagenesis, Site-Directed/methods
  • Protein Engineering/methods*
  • Reverse Genetics/methods*
  • Zebrafish/genetics*
  • Zinc Fingers
PubMed: 21924156 Full text @ Meth. Cell. Biol.
ABSTRACT
Zinc finger nucleases provide an important platform for performing reverse genetics in zebrafish. Here we review various methods and resources that have been used to create customized zinc finger nucleases for use in zebrafish. We also provide a framework for choosing among the various publicly available platforms available to engineer ZFNs.
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