PUBLICATION

Smyd3 is required for the development of cardiac and skeletal muscle in zebrafish

Authors
Fujii, T., Tsunesumi, S., Yamaguchi, K., Watanabe, S., and Furukawa, Y.
ID
ZDB-PUB-110907-24
Date
2011
Source
PLoS One   6(8): e23491 (Journal)
Registered Authors
Watanabe, Sumiko
Keywords
Embryos, Zebrafish, Skeletal muscles, Heart, Muscle differentiation, Transcription factors, Histones, Edema
MeSH Terms
  • Animals
  • Biomarkers/metabolism
  • Embryo, Nonmammalian/abnormalities
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Gene Knockdown Techniques
  • Heart/drug effects
  • Heart/embryology*
  • Histone-Lysine N-Methyltransferase/genetics
  • Histone-Lysine N-Methyltransferase/metabolism*
  • In Situ Hybridization
  • Morpholinos/pharmacology
  • Muscle Development/drug effects
  • Muscle, Skeletal/abnormalities
  • Muscle, Skeletal/drug effects
  • Muscle, Skeletal/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
21887258 Full text @ PLoS One
Abstract
Modifications of histone tails are involved in the regulation of a wide range of biological processes including cell cycle, cell survival, cell division, and cell differentiation. Among the modifications, histone methylation plays a critical role in cardiac and skeletal muscle differentiation. In our earlier studies, we found that SMYD3 has methyltransferase activity to histone H3 lysine 4, and that its up-regulation is involved in the tumorigenesis of human colon, liver, and breast. To clarify the role of Smyd3 in development, we have studied its expression patterns in zebrafish embryos and the effect of its suppression on development using Smyd3-specific antisense morpholino-oligonucleotides. We here show that transcripts of smyd3 were expressed in zebrafish embryos at all developmental stages examined and that knockdown of smyd3 in embryos resulted in pericardial edema and defects in the trunk structure. In addition, these phenotypes were associated with abnormal expression of three heart-chamber markers including cmlc2, amhc and vmhc, and abnormal expression of myogenic regulatory factors including myod and myog. These data suggest that Smyd3 plays an important role in the development of heart and skeletal muscle.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping