PUBLICATION
Proteome profile of zebrafish kidney
- Authors
- Saxena, S., Singh, S.K., Lakshmi, M.G., Meghah, V., Sundaram, C.S., Swamy, C.V., and Idris, M.M.
- ID
- ZDB-PUB-110816-25
- Date
- 2011
- Source
- Journal of proteomics 74(12): 2937-47 (Journal)
- Registered Authors
- Idris, Mohammed, Singh, Sachin
- Keywords
- zebrafish, kidney, proteome, network pathway
- MeSH Terms
-
- Animals
- Biomarkers/metabolism
- Kidney/metabolism*
- Kidney Diseases/metabolism
- Proteome/metabolism*
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism*
- PubMed
- 21839869 Full text @ J. Proteomics
Citation
Saxena, S., Singh, S.K., Lakshmi, M.G., Meghah, V., Sundaram, C.S., Swamy, C.V., and Idris, M.M. (2011) Proteome profile of zebrafish kidney. Journal of proteomics. 74(12):2937-47.
Abstract
The most imperative organ, kidney has been widely studied in zebrafish for its simplified structures and development. Understanding the proteomic component of kidney might lead to a better insight for understanding the structural and functional complexity of kidney. In this study we have analyzed the proteome profile of the zebrafish kidney based on gel based proteome mapping techniques involving single dimension gel electrophoresis nanoflow liquid chromatography mass spectrophotometer, single dimension gel electrophoresis microflow ESI liquid chromatography mass spectrophotometer and two dimensional gel electrophoresis matrix assisted laser desorption/ionization assay mass spectrophotometer analysis. A total of 385 proteins were identified consensually from the analysis as zebrafish kidney specific protein which includes 313, 55, and 87 proteins identified based on 1-DE FTMS/ITMSMS, 1-DE ESI-LCMS/MS and 2-DE MALDI MS/MS approaches respectively. The identified kidney proteome dataset was found to be representatives of diverse pI, mass, localization, process and functions. The kidney proteome dataset was found to be significantly associated with various metabolic, catabolic, cytoskeleton remodeling and rectal disease pathways. The engendered kidney protein catalog will serve as a template for understanding kidney functions and biomarker identification related to different kidney disorders.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping