ZFIN ID: ZDB-PUB-110713-75
Lessons from morpholino-based screening in zebrafish
Bedell, V.M., Westcot, S.E., and Ekker, S.C.
Date: 2011
Source: Briefings in functional genomics & proteomics 10(4): 181-8 (Journal)
Registered Authors: Bedell, Victoria, Ekker, Stephen C., Westcot, Stephanie
Keywords: morpholinos, zebrafish, knockdown
MeSH Terms: Animals; Computational Biology; Genetic Testing*; Oligonucleotides, Antisense/pharmacology*; Phenotype (all 7) expand
PubMed: 21746693 Full text @ Brief. Funct. Genomic. Proteomic.
ABSTRACT
Morpholino oligonucleotides (MOs) are an effective, gene-specific antisense knockdown technology used in many model systems. Here we describe the application of MOs in zebrafish (Danio rerio) for in vivo functional characterization of gene activity. We summarize our screening experience beginning with gene target selection. We then discuss screening parameter considerations and data and database management. Finally, we emphasize the importance of off-target effect management and thorough downstream phenotypic validation. We discuss current morpholino limitations, including reduced stability when stored in aqueous solution. Advances in MO technology now provide a measure of spatiotemporal control over MO activity, presenting the opportunity for incorporating more finely tuned analyses into MO-based screening. Therefore, with careful management, MOs remain a valuable tool for discovery screening as well as individual gene knockdown analysis.
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