PUBLICATION
Lessons from morpholino-based screening in zebrafish
- Authors
- Bedell, V.M., Westcot, S.E., and Ekker, S.C.
- ID
- ZDB-PUB-110713-75
- Date
- 2011
- Source
- Briefings in functional genomics & proteomics 10(4): 181-8 (Journal)
- Registered Authors
- Bedell, Victoria, Ekker, Stephen C., Westcot, Stephanie
- Keywords
- morpholinos, zebrafish, knockdown
- MeSH Terms
-
- Animals
- Computational Biology
- Genetic Testing*
- Oligonucleotides, Antisense/pharmacology*
- Phenotype
- Reproducibility of Results
- Zebrafish/genetics*
- PubMed
- 21746693 Full text @ Brief. Funct. Genomic. Proteomic.
Citation
Bedell, V.M., Westcot, S.E., and Ekker, S.C. (2011) Lessons from morpholino-based screening in zebrafish. Briefings in functional genomics & proteomics. 10(4):181-8.
Abstract
Morpholino oligonucleotides (MOs) are an effective, gene-specific antisense knockdown technology used in many model systems.
Here we describe the application of MOs in zebrafish (Danio rerio) for in vivo functional characterization of gene activity. We summarize our screening experience beginning with gene target selection.
We then discuss screening parameter considerations and data and database management. Finally, we emphasize the importance
of off-target effect management and thorough downstream phenotypic validation. We discuss current morpholino limitations,
including reduced stability when stored in aqueous solution. Advances in MO technology now provide a measure of spatiotemporal
control over MO activity, presenting the opportunity for incorporating more finely tuned analyses into MO-based screening.
Therefore, with careful management, MOs remain a valuable tool for discovery screening as well as individual gene knockdown
analysis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping