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ZFIN ID: ZDB-PUB-110628-17
Molecular Cloning and mRNA Expression of the Liver-Specific Cathepsin L1 Gene of the Olive Flounder, Paralichthys olivaceus
Kim, Y.O., Park, E.M., Seo, J.S., Nam, B.H., Kong, H.J., Kim, W.J., Kim, B.S., Kim, K.K., and Lee, S.J.
Date: 2011
Source: Bioscience, biotechnology, and biochemistry   75(6): 1214-1218 (Journal)
Registered Authors:
Keywords: paralichthys olvaceus, cathepsin L, gene expression, RT-PCR, in situ hybridization
MeSH Terms:
  • Amino Acid Motifs
  • Animals
  • Cathepsin L/genetics
  • Cathepsin L/metabolism*
  • Cloning, Molecular
  • Escherichia coli
  • Fish Proteins/genetics
  • Fish Proteins/metabolism*
  • Flounder/genetics
  • Flounder/metabolism*
  • Gene Expression
  • Gene Library
  • Humans
  • Liver/metabolism*
  • Molecular Sequence Data
  • Phylogeny
  • Plasmids
  • RNA, Messenger
  • Recombinant Proteins/genetics
  • Recombinant Proteins/metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Transformation, Bacterial
PubMed: 21670505 Full text @ Biosci. Biotechnol. Biochem.
We isolated a homolog of cathepsin L from a cDNA library of the olive flounder liver. The flounder cathepsin L1 transcript consisted of 1,221 bp that encoded a polypeptide of 334 amino acids. The overall identity between flounder cathepsin L1 and other cathepsin Ls was 50–64%, and flounder cathepsin L1 contained the highly conserved ERFNIN-motif. A phylogenetic tree indicated that flounder cathepsin L1 is in the same monophyletic group as zebrafish cathepsin Lc. RT-PCR analysis revealed that cathepsin L1 transcripts were expressed only in the liver. They were detected from 28 d post-hatching. Under starvation conditions, cathepsin L1 expression was decreased at 30 d.