PUBLICATION

Transplantation of cells directly into the kidney of adult zebrafish

Authors
Diep, C.Q., and Davidson, A.J.
ID
ZDB-PUB-110609-49
Date
2011
Source
Journal of visualized experiments : JoVE   (51): (Journal)
Registered Authors
Davidson, Alan
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Hematopoietic Stem Cell Transplantation/methods
  • Hematopoietic Stem Cells/cytology
  • Kidney/cytology
  • Kidney/surgery*
  • Male
  • Regenerative Medicine/methods*
  • Zebrafish
PubMed
21633330 Full text @ J. Vis. Exp.
Abstract
Regenerative medicine based on the transplantation of stem or progenitor cells into damaged tissues has the potential to treat a wide range of chronic diseases1. However, most organs are not easily accessible, necessitating the need to develop surgical methods to gain access to these structures. In this video article, we describe a method for transplanting cells directly into the kidney of adult zebrafish, a popular model to study regeneration and disease2. Recipient fish are pre-conditioned by irradiation to suppress the immune rejection of the injected cells3. We demonstrate how the head kidney can be exposed by a lateral incision in the flank of the fish, followed by the injection of cells directly in to the organ. Using fluorescently labeled whole kidney marrow cells comprising a mixed population of renal and hematopoietic precursors, we show that nephron progenitors can engraft and differentiate into new renal tissue - the gold standard of any cell-based regenerative therapy. This technique can be adapted to deliver purified stem or progenitor cells and/or small molecules to the kidney as well as other internal organs and further enhances the zebrafish as a versatile model to study regenerative medicine.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes
Video article + print.