PUBLICATION
Optogenetics: A new enlightenment age for zebrafish neurobiology
- Authors
- Del Bene, F., and Wyart, C.
- ID
- ZDB-PUB-110524-26
- Date
- 2012
- Source
- Developmental Neurobiology 72(3): 404-414 (Review)
- Registered Authors
- Del Bene, Filippo, Wyart, Claire
- Keywords
- none
- MeSH Terms
-
- Animals
- Genetic Techniques/trends*
- Humans
- Molecular Imaging/trends*
- Nerve Net/chemistry
- Nerve Net/physiology*
- Neurons/chemistry
- Neurons/physiology
- Opsins/physiology
- Optical Phenomena*
- Photic Stimulation/methods
- Zebrafish/physiology*
- PubMed
- 21567983 Full text @ Dev. Neurobiol.
Citation
Del Bene, F., and Wyart, C. (2012) Optogenetics: A new enlightenment age for zebrafish neurobiology. Developmental Neurobiology. 72(3):404-414.
Abstract
Zebrafish became a model of choice for neurobiology due to the transparency of its brain and because of its amenability to genetic manipulation. In particular, at early stages of development the intact larva is an ideal system to apply optical techniques for deep imaging in the nervous system, as well as genetically encoded tools for targeting subsets of neurons and monitoring and manipulating their activity. For these applications, new genetically encoded optical tools, fluorescent sensors and light-gated channels, have been generated, creating the field of "optogenetics". It is now possible to monitor and control neuronal activity with minimal perturbation and unprecedented spatio-temporal resolution. We describe here the main achievements that have occurred in the last decade in imaging and manipulating neuronal activity in intact zebrafish larvae. We provide also examples of functional dissection of neuronal circuits achieved with the applications of these techniques in the visual and locomotor systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping