PUBLICATION
Epithelial cell extrusion requires the sphingosine-1-phosphate receptor 2 pathway
- Authors
- Gu, Y., Forostyan, T., Sabbadini, R., and Rosenblatt, J.
- ID
- ZDB-PUB-110523-22
- Date
- 2011
- Source
- The Journal of cell biology 193(4): 667-676 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Adrenal Gland Diseases/diagnosis*
- Adrenal Glands/pathology
- Aged
- Cysts/diagnosis*
- Female
- Humans
- Tomography, X-Ray Computed
- Ultrasonography
- PubMed
- 21555463 Full text @ J. Cell Biol.
Citation
Gu, Y., Forostyan, T., Sabbadini, R., and Rosenblatt, J. (2011) Epithelial cell extrusion requires the sphingosine-1-phosphate receptor 2 pathway. The Journal of cell biology. 193(4):667-676.
Abstract
To maintain an intact barrier, epithelia eliminate dying cells by extrusion. During extrusion, a cell destined for apoptosis signals its neighboring cells to form and contract a ring of actin and myosin, which squeezes the dying cell out of the epithelium. Here, we demonstrate that the signal produced by dying cells to initiate this process is sphingosine-1-phosphate (S1P). Decreasing S1P synthesis by inhibiting sphingosine kinase activity or by blocking extracellular S1P access to its receptor prevented apoptotic cell extrusion. Extracellular S1P activates extrusion by binding the S1P(2) receptor in the cells neighboring a dying cell, as S1P(2) knockdown in these cells or its loss in a zebrafish mutant disrupted cell extrusion. Because live cells can also be extruded, we predict that this S1P pathway may also be important for driving delamination of stem cells during differentiation or invasion of cancer cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping