ZFIN ID: ZDB-PUB-110518-51
The use of RNAi technologies for gene knockdown in zebrafish
Kelly, A., and Hurlstone, A.F.
Date: 2011
Source: Briefings in functional genomics & proteomics   10(4): 189-96 (Review)
Registered Authors: Hurlstone, Adam
Keywords: RNA interference, zebrafish, siRNA, dsRNA, vector-mediated RNAi, Argonaute 2
MeSH Terms:
  • Animals
  • Gene Knockdown Techniques/methods*
  • Genetic Vectors/genetics
  • RNA Interference*
  • RNA, Double-Stranded/metabolism
  • RNA, Small Interfering/metabolism
  • Zebrafish/genetics*
PubMed: 21525144 Full text @ Brief. Funct. Genomic. Proteomic.
Despite being a popular and versatile model organism in which to study development and model disease, the use of zebrafish has been hampered by the lack of a reliable, stable and cost-effective method of gene knockdown. It is therefore not surprising that the discovery of RNAi as an exploitable method of post-transcriptional gene regulation has created a lot of excitement within the zebrafish research community. However, despite concerted efforts in the field, progress in the use of RNAi technologies in zebrafish has been extremely slow and a reliable means of RNAi-mediated gene knockdown remains elusive. The following reviews progress in the field to date, highlights the major pitfalls identified and suggests possible future directions.