PUBLICATION
The use of RNAi technologies for gene knockdown in zebrafish
- Authors
- Kelly, A., and Hurlstone, A.F.
- ID
- ZDB-PUB-110518-51
- Date
- 2011
- Source
- Briefings in functional genomics & proteomics 10(4): 189-96 (Review)
- Registered Authors
- Hurlstone, Adam
- Keywords
- RNA interference, zebrafish, siRNA, dsRNA, vector-mediated RNAi, Argonaute 2
- MeSH Terms
-
- Animals
- Gene Knockdown Techniques/methods*
- Genetic Vectors/genetics
- RNA Interference*
- RNA, Double-Stranded/metabolism
- RNA, Small Interfering/metabolism
- Zebrafish/genetics*
- PubMed
- 21525144 Full text @ Brief. Funct. Genomic. Proteomic.
Citation
Kelly, A., and Hurlstone, A.F. (2011) The use of RNAi technologies for gene knockdown in zebrafish. Briefings in functional genomics & proteomics. 10(4):189-96.
Abstract
Despite being a popular and versatile model organism in which to study development and model disease, the use of zebrafish has been hampered by the lack of a reliable, stable and cost-effective method of gene knockdown. It is therefore not surprising that the discovery of RNAi as an exploitable method of post-transcriptional gene regulation has created a lot of excitement within the zebrafish research community. However, despite concerted efforts in the field, progress in the use of RNAi technologies in zebrafish has been extremely slow and a reliable means of RNAi-mediated gene knockdown remains elusive. The following reviews progress in the field to date, highlights the major pitfalls identified and suggests possible future directions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping