|ZFIN ID: ZDB-PUB-110518-51|
The use of RNAi technologies for gene knockdown in zebrafish
Kelly, A., and Hurlstone, A.F.
|Source:||Briefings in functional genomics & proteomics 10(4): 189-96 (Review)|
|Registered Authors:||Hurlstone, Adam|
|Keywords:||RNA interference, zebrafish, siRNA, dsRNA, vector-mediated RNAi, Argonaute 2|
|PubMed:||21525144 Full text @ Brief. Funct. Genomic. Proteomic.|
Kelly, A., and Hurlstone, A.F. (2011) The use of RNAi technologies for gene knockdown in zebrafish. Briefings in functional genomics & proteomics. 10(4):189-96.
ABSTRACTDespite being a popular and versatile model organism in which to study development and model disease, the use of zebrafish has been hampered by the lack of a reliable, stable and cost-effective method of gene knockdown. It is therefore not surprising that the discovery of RNAi as an exploitable method of post-transcriptional gene regulation has created a lot of excitement within the zebrafish research community. However, despite concerted efforts in the field, progress in the use of RNAi technologies in zebrafish has been extremely slow and a reliable means of RNAi-mediated gene knockdown remains elusive. The following reviews progress in the field to date, highlights the major pitfalls identified and suggests possible future directions.
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