ZFIN ID: ZDB-PUB-110316-15
Regulation of hub mRNA stability and translation by miR430 and the dead end protein promotes preferential expression in zebrafish primordial germ cells
Mickoleit, M., Banisch, T.U., and Raz, E.
Date: 2011
Source: Developmental dynamics : an official publication of the American Association of Anatomists   240(3): 695-703 (Journal)
Registered Authors: Banisch, Torsten, Mickoleit, Michaela, Raz, Erez
Keywords: Hu protein, HuB, germline, PGC, miRNA, zebrafish, dead end
MeSH Terms:
  • Animals
  • ELAV Proteins/genetics*
  • ELAV Proteins/metabolism*
  • Embryo, Nonmammalian/metabolism*
  • Flow Cytometry
  • Germ Cells/cytology*
  • Germ Cells/metabolism*
  • In Situ Hybridization
  • MicroRNAs/genetics*
  • Microscopy, Fluorescence
  • Polymerase Chain Reaction
  • RNA Stability/genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 21337467 Full text @ Dev. Dyn.
The Hu proteins are RNA-binding proteins known to be involved in various aspects of RNA metabolism, such as nucleo-cytoplasmic shuttling, translation, and stability. These proteins are predominantly expressed in neuronal tissues and are important for neuronal differentiation and plasticity. Here, we report on the regulation over hub mRNA stability and function in zebrafish embryos. Using reporters encoding for fluorescent proteins, we show that hub RNA is a target of global miRNA-mediated repression, while the RNA-binding protein Dead end (Dnd) contributes to maintenance of the expression in the primordial germ cells (PGCs).