PUBLICATION

Cofilin regulator 14-3-3{zeta} is an evolutionarily conserved protein required for phagocytosis and microbial resistance

Authors
Ulvila, J., Vanha-Aho, L.M., Kleino, A., Vähä-Mäkilä, M., Vuoksio, M., Eskelinen, S., Hultmark, D., Kocks, C., Hallman, M., Parikka, M., and Rämet, M.
ID
ZDB-PUB-110110-33
Date
2011
Source
Journal of Leukocyte Biology   89(5): 649-59 (Journal)
Registered Authors
Keywords
Drosophila, zebrafish, plasmatocyte, macrophage, immunity
MeSH Terms
  • 14-3-3 Proteins/antagonists & inhibitors
  • 14-3-3 Proteins/genetics*
  • Actin Depolymerizing Factors/genetics*
  • Animals
  • Biological Evolution*
  • Cells, Cultured
  • Drosophila melanogaster/genetics*
  • Drosophila melanogaster/immunology
  • Drosophila melanogaster/microbiology*
  • Escherichia coli/pathogenicity*
  • Escherichia coli Infections/genetics
  • Escherichia coli Infections/immunology
  • Escherichia coli Infections/microbiology
  • Female
  • Gene Silencing
  • Genes, Insect
  • Hemocytes/metabolism
  • Male
  • Mice
  • Phagocytosis/physiology*
  • RNA, Small Interfering/genetics
  • Zebrafish/genetics
  • Zebrafish/immunology
  • Zebrafish/microbiology
PubMed
21208897 Full text @ J. Leukoc. Biol.
Abstract
Phagocytosis is an ancient cellular process that plays an important role in host defense. In Drosophila melanogaster phagocytic, macrophage-like hemocytes recognize and ingest microbes. We performed an RNAi-based in vitro screen in the Drosophila hemocyte cell line S2 and identified Abi, cpa, cofilin regulator 14-3-3ζ, tlk, CG2765, and CG15609 as mediators of bacterial phagocytosis. Of these identified genes, 14-3-3ζ had an evolutionarily conserved role in phagocytosis: bacterial phagocytosis was compromised when 14-3-3ζ was targeted with RNAi in primary Drosophila hemocytes and when the orthologous genes Ywhab and Ywhaz were silenced in zebrafish and mouse RAW 264.7 cells, respectively. In Drosophila and zebrafish infection models, 14-3-3ζ was required for resistance against Staphylococcus aureus. We conclude that 14-3-3ζ is essential for phagocytosis and microbial resistance in insects and vertebrates.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping