PUBLICATION
Unpredictable chronic stress model in Zebrafish (Danio rerio): Behavioral and physiological responses
- Authors
- Piato, A.L., Capiotti, K.M., Tamborski, A.R., Oses, J.P., Barcellos, L.J., Bogo, M.R., Lara, D.R., Vianna, M.R., and Bonan, C.D.
- ID
- ZDB-PUB-110103-26
- Date
- 2011
- Source
- Progress in neuro-psychopharmacology & biological psychiatry 35(2): 561-567 (Journal)
- Registered Authors
- Bonan, Carla Denise, Vianna, Mônica Ryff Moreira Roca
- Keywords
- zebrafish, unpredictable chronic stress protocol (UCS), anxiety, depression, cortisol
- MeSH Terms
-
- Animals
- Anxiety/psychology*
- Behavior, Animal*
- Corticotropin-Releasing Hormone/genetics
- Exploratory Behavior/physiology*
- Hydrocortisone/analysis
- Hydrocortisone/physiology
- Male
- Memory, Long-Term/physiology
- Models, Animal*
- Motor Activity/physiology
- Receptors, Glucocorticoid/genetics
- Stress, Physiological/physiology*
- Stress, Psychological/physiopathology*
- Zebrafish*
- PubMed
- 21187119 Full text @ Prog. Neuropsychopharmacol. Biol. Psychiatry
Citation
Piato, A.L., Capiotti, K.M., Tamborski, A.R., Oses, J.P., Barcellos, L.J., Bogo, M.R., Lara, D.R., Vianna, M.R., and Bonan, C.D. (2011) Unpredictable chronic stress model in Zebrafish (Danio rerio): Behavioral and physiological responses. Progress in neuro-psychopharmacology & biological psychiatry. 35(2):561-567.
Abstract
Zebrafish (Danio rerio) have emerged as a promising model organism to study development, toxicology, pharmacology, and neuroscience, among other areas. Despite the increasing number of studies using zebrafish, behavioral studies with this species are still elementary when compared to rodents. The aim of this study was to develop a model of unpredictable chronic stress (UCS) in zebrafish. We evaluated the effects of UCS protocol during 7 or 14days on behavioral and physiological parameters. The effects of stress were evaluated in relation to anxiety and exploratory behavior, memory, expression of corticotrophin-releasing factor (CRF) and glucocorticoid receptor (GR), and cortisol levels. As expected, UCS protocol increased the anxiety levels, impaired cognitive function, and increased CRF while decreased GR expression. Moreover, zebrafish submitted to 7 or 14days of UCS protocol presented increased cortisol levels. The protocol developed here is a complementary model for studying the neurobiology and the effects of chronic stress in behavioral and physiological parameters. In addition, this protocol is less time consuming than standard rodent models commonly used to study chronic stress. These results confirm UCS in zebrafish as an adequate model to preclinical studies of stress, although further studies are warranted to determine its predictive validity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping