PUBLICATION
Molecular Cloning and Expression Analysis of P-Selectin from Zebrafish (Danio rerio)
- Authors
- Sun, G., Pan, J., Liu, K., Wang, X., and Wang, S.
- ID
- ZDB-PUB-101222-19
- Date
- 2010
- Source
- International Journal of Molecular Sciences 11(11): 4618-4630 (Journal)
- Registered Authors
- Keywords
- P-selectin, zebrafish, cloning, expression, adenosine diphosphate
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Conserved Sequence
- Gene Expression Regulation, Developmental*
- Molecular Sequence Data
- P-Selectin/chemistry
- P-Selectin/genetics
- P-Selectin/metabolism*
- Protein Structure, Tertiary
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Zebrafish
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 21151460 Full text @ Int. J. Mol. Sci.
Citation
Sun, G., Pan, J., Liu, K., Wang, X., and Wang, S. (2010) Molecular Cloning and Expression Analysis of P-Selectin from Zebrafish (Danio rerio). International Journal of Molecular Sciences. 11(11):4618-4630.
Abstract
The glycoprotein P-selectin belongs to the selectin family of cell adhesion molecules. In this study, we cloned the full-length cDNA of P-selectin from zebrafish (Danio rerio) by the method of rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). Zebrafish P-selectin cDNA is 2,800 bp and encodes a putative 868 amino acid protein with a theoretical molecular weight of 122.36 kDa and isoelectric point of 6.27. A signal peptide of 25 amino acids is predicted at the N-terminus of the putative protein. All structural domains involved in P-selectin function are conserved in the putative protein. The amino acid sequence of zebrafish P-selectin is 37% to 39% identical to that of mammalian P-selectins. Real-time quantitative PCR and whole-mount in situ hybridization analysis revealed that P-selectin was expressed in early embryonic development, the expression increased from 0.2 hpf (1-cell stage) to 72 hpf, and the expression significantly upregulated within 30 minutes of ADP induction. The results indicate that the structure of P-selectin protein is highly conserved among species and zebrafish P-selectin plays an important role in early embryonic development and probably has similar biological function to mammalian P-selectins.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping