ZFIN ID: ZDB-PUB-101213-1
Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish
Geudens, I., Herpers, R., Hermans, K., Segura, I., Ruiz de Almodovar, C., Bussmann, J., De Smet, F., Vandevelde, W., Hogan, B.M., Siekmann, A., Claes, F., Moore, J.C., Pistocchi, A.S., Loges, S., Mazzone, M., Mariggi, G., Bruyere, F., Cotelli, F., Kerjaschki, D., Noel, A., Foidart, J.M., Gerhardt, H., Ny, A., Langenberg, T., Lawson, N.D., Duckers, H.J., Schulte-Merker, S., Carmeliet, P., and Dewerchin, M.
Date: 2010
Source: Arterioscler. Thromb. Vasc. Biol. 30(9): 1695-1702 (Journal)
Registered Authors: Bussmann, Jeroen, Cotelli, Franco, Herpers, Robert, Hogan, Ben M., Langenberg, Tobias, Lawson, Nathan, Moore, John, Schulte-Merker, Stefan, Siekmann, Arndt Friedrich
Keywords: vascular biology, gene silencing, lymphangiogenesis, notch signaling, zebrafish
MeSH Terms: Animals; Animals, Genetically Modified; Biomarkers/metabolism; COS Cells; Cell Movement; Cell Proliferation; Cercopithecus aethiops; Coculture Techniques; Embryo, Nonmammalian/metabolism; Endothelial Cells/metabolism; Gene Expression Regulation, Developmental; Gene Knockdown Techniques; Gene Silencing; Humans; Intracellular Signaling Peptides and Proteins; Luminescent Proteins/biosynthesis; Luminescent Proteins/genetics; Lymphangiogenesis*/genetics; Lymphatic System/embryology; Lymphatic System/metabolism*; Membrane Proteins/genetics; Membrane Proteins/metabolism*; RNA, Messenger/metabolism; Receptors, Notch/genetics; Receptors, Notch/metabolism*; Signal Transduction*; Thoracic Duct/embryology; Thoracic Duct/metabolism; Zebrafish/embryology; Zebrafish/genetics; Zebrafish/metabolism*; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism*
PubMed: 20466977 Full text @ Arterioscler. Thromb. Vasc. Biol.
FIGURES   (current status)
ABSTRACT
OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.
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