PUBLICATION
Chapter 7 - Photoreceptor Structure and Development Analyses using GFP Transgenes
- Authors
- Perkins, B.D., and Fadool, J.M.
- ID
- ZDB-PUB-101201-29
- Date
- 2010
- Source
- Methods in cell biology 100: 205-218 (Chapter)
- Registered Authors
- Fadool, James M., Perkins, Brian
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Gene Transfer Techniques*
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Photoreceptor Cells/cytology*
- Photoreceptor Cells/metabolism
- Retina/cytology*
- Retina/metabolism
- Zebrafish*
- PubMed
- 21111218 Full text @ Meth. Cell. Biol.
Citation
Perkins, B.D., and Fadool, J.M. (2010) Chapter 7 - Photoreceptor Structure and Development Analyses using GFP Transgenes. Methods in cell biology. 100:205-218.
Abstract
In recent years, studies of zebrafish rod and cone photoreceptors have yielded novel insights into the differentiation of distinct photoreceptor cell types and the mechanisms guiding photoreceptor regeneration following cell death, and they have provided models of human retinal degeneration. These studies were facilitated by the use of transgenic zebrafish expressing fluorescent reporter genes under the control of various cell-specific promoters. Improvements in transgenesis techniques (e.g., Tol2 transposition), the availability of numerous fluorescent reporter genes with different localization properties, and the ability to generate transgenes via recombineering (e.g., Gateway technology) have enabled researchers to quickly develop transgenic lines that improve our understanding of the causes of human blindness and ways to mitigate its effects.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping