PUBLICATION
No bioavailability of 17α-ethinylestradiol when associated with nC60 aggregates during dietary exposure in adult male zebrafish (Danio rerio)
- Authors
- Park, J.W., Henry, T.B., Menn, F.M., Compton, R.N., and Sayler, G.
- ID
- ZDB-PUB-101018-22
- Date
- 2010
- Source
- Chemosphere 81(10): 1227-1232 (Journal)
- Registered Authors
- Henry, Theodore B.
- Keywords
- C60 fullerene, EE2, Dietary exposure, Bioavailability, Zebrafish
- MeSH Terms
-
- Administration, Oral
- Animals
- Artemia/drug effects
- Artemia/metabolism
- Biological Availability
- Diet
- Ethinyl Estradiol/chemistry
- Ethinyl Estradiol/pharmacokinetics*
- Ethinyl Estradiol/toxicity
- Fullerenes/chemistry
- Fullerenes/pharmacokinetics*
- Fullerenes/toxicity
- Liver/drug effects
- Liver/metabolism
- Male
- Vitellogenins/genetics
- Vitellogenins/metabolism
- Water Pollutants, Chemical/chemistry
- Water Pollutants, Chemical/pharmacokinetics*
- Water Pollutants, Chemical/toxicity
- Zebrafish/metabolism
- PubMed
- 20937515 Full text @ Chemosphere
Citation
Park, J.W., Henry, T.B., Menn, F.M., Compton, R.N., and Sayler, G. (2010) No bioavailability of 17α-ethinylestradiol when associated with nC60 aggregates during dietary exposure in adult male zebrafish (Danio rerio). Chemosphere. 81(10):1227-1232.
Abstract
The C(60) fullerene is a manufactured carbon nanoparticle (CNP) that could pose a risk to humans and other organisms after release into the environment. In surface waters, C(60) is likely to be present as aggregates of nC(60) and these aggregates can associate with other substances that are toxic. Our goal was to evaluate the association of a model contaminant [17α-ethinylestradiol (EE2)] with nC(60) and determine bioavailability of EE2 after accumulation by a filter feeding organism [Brine shrimp (BS) Artemia sp.] and subsequent dietary exposure in zebrafish. Aqueous suspensions of nC(60) were prepared (600mg C(60)/900mL, 6-month water stirred method) with/without EE2 (1μg/L) and BS were exposed to these preparations. Accumulation of nC(60) in gut of BS was assessed by light microscopy, and C(60) were extracted from BS and concentration analyzed by HPLC. Adult male zebrafish were fed (5d) live BS according to the following treatments: BS (control); BS containing nC(60); BS containing nC(60)+EE2; or BS containing EE2. Liver was excised from exposed fish and total RNA was extracted for assessment of vitellogenin gene (vtg1A/B) expression. The vtg1A/B was highly up-regulated in fish exposed to BS containing EE2, but expression of vtg1A/B did not differ from controls in other treatments. The EE2 associated with nC(60) did not become bioavailable in zebrafish during passage through the intestinal tract of zebrafish. Results have implications on the effect of nC(60) on the bioavailability of co-contaminants in organisms during dietary exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping