ZFIN ID: ZDB-PUB-100910-14 |
Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis
Barresi, M.J., Burton, S., Dipietrantonio, K., Amsterdam, A., Hopkins, N., and Karlstrom, R.O.
Date: | 2010 |
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Source: | Developmental dynamics : an official publication of the American Association of Anatomists 239(10): 2603-2618 (Journal) |
Registered Authors: | Amsterdam, Adam, Barresi, Michael J. F., Hopkins, Nancy, Karlstrom, Rolf |
Keywords: | zebrafish, insertional mutants, axon guidance, astroglia, radial glia, glia, POC, chiasm, motor axon, hindbrain, neurogenesis, gliogenesis, Gfap |
MeSH Terms: |
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PubMed: | 20806318 Full text @ Dev. Dyn. |
Citation
Barresi, M.J., Burton, S., Dipietrantonio, K., Amsterdam, A., Hopkins, N., and Karlstrom, R.O. (2010) Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis. Developmental dynamics : an official publication of the American Association of Anatomists. 239(10):2603-2618.
ABSTRACT
The formation of the central nervous system depends on the coordinated development of neural and glial cell types that arise from a common precursor. Using an existing group of zebrafish mutants generated by viral insertion, we performed a "shelf-screen" to identify genes necessary for astroglial development and axon scaffold formation. We screened 274 of 315 viral insertion lines using antibodies that label axons (anti-Acetylated Tubulin) and astroglia (anti-Gfap) and identified 25 mutants with defects in gliogenesis, glial patterning, neurogenesis, and axon guidance. We also identified a novel class of mutants affecting radial glial cell numbers. Defects in astroglial patterning were always associated with axon defects, supporting an important role for axon-glial interactions during axon scaffold development. The genes disrupted in these viral lines have all been identified, providing a powerful new resource for the study of axon guidance, glio- and neurogenesis, and neuron-glial interactions during development of the vertebrate CNS.
ADDITIONAL INFORMATION
- Genes / Markers (27)
- Antibodies (2)
- Expression and Phenotype Data
- Mutations and Transgenics (29)
- Fish (56)