PUBLICATION
Molecular characterization of atrogin-1/F-box protein-32 (FBXO32) and F-box protein-25 (FBXO25) in rainbow trout (Oncorhynchus mykiss): Expression across tissues in response to feed deprivation
- Authors
- Cleveland, B.M., and Evenhuis, J.P.
- ID
- ZDB-PUB-100719-12
- Date
- 2010
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 157(3): 248-257 (Journal)
- Registered Authors
- Keywords
- Atrogin-1, Atrophy, Starvation, Ubiquitin ligase, Salmonid, Protein degradation, Fish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- F-Box Proteins/genetics
- F-Box Proteins/metabolism*
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Food Deprivation
- Gene Expression
- Humans
- Mice
- Molecular Sequence Data
- Oncorhynchus mykiss/genetics
- Oncorhynchus mykiss/metabolism*
- Phylogeny
- Protein Structure, Tertiary
- SKP Cullin F-Box Protein Ligases/genetics
- SKP Cullin F-Box Protein Ligases/metabolism*
- Sequence Alignment
- Tissue Distribution
- PubMed
- 20601059 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Cleveland, B.M., and Evenhuis, J.P. (2010) Molecular characterization of atrogin-1/F-box protein-32 (FBXO32) and F-box protein-25 (FBXO25) in rainbow trout (Oncorhynchus mykiss): Expression across tissues in response to feed deprivation. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 157(3):248-257.
Abstract
The characteristic increase in protein catabolism during muscle atrophy is largely the result of an increase in E3 ubiquitin ligase expression, specifically that of atrogin-1, or FBXO32, which functions to polyubiquitinate proteins. In rainbow trout, the cDNA sequences of two E3 ubiquitin ligase F-box proteins, FBXO32 and FBXO25, were characterized and their expression across tissues in response to feed deprivation was determined. The cDNA sequence for FBXO32 encodes a protein 355 amino acids long and is 97% identical to the homologous protein in salmon, 85% to zebrafish and 72% identical to both human and mouse. The cDNA for FBXO25 encodes a protein 356 amino acids in length that is 98% identical to the homologous protein in salmon, 84% to zebrafish, and 75% to human. After 28days of feed deprivation, FBXO32 expression increased by approximately 13-fold, 3-fold, and 5-fold in white muscle, red muscle, and intestine, respectively (P<0.05). Expression of FBXO32 and FBXO25 in kidney decreased 0.3-fold and 0.2-fold, respectively, and FBXO25 expression decreased by 0.2-fold in liver (P<0.05). These results indicate that these protein sequences are conserved and suggest that the up-regulation of FBXO32 is associated with skeletal and smooth muscle atrophy that occurs during fasting.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping