PUBLICATION
Single-cell analysis of somatotopic map formation in the zebrafish lateral line system
- Authors
- Sato, A., Koshida, S., and Takeda, H.
- ID
- ZDB-PUB-100621-22
- Date
- 2010
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(7): 2058-2065 (Journal)
- Registered Authors
- Koshida, Sumito, Takeda, Hiroyuki
- Keywords
- lateral line, zebrafish, somatotopy, topographic map, single-cell, live imaging, neural circuit formation
- MeSH Terms
-
- Animals
- Lateral Line System/cytology
- Lateral Line System/embryology*
- Neurons/cytology
- Zebrafish/embryology*
- PubMed
- 20549741 Full text @ Dev. Dyn.
Citation
Sato, A., Koshida, S., and Takeda, H. (2010) Single-cell analysis of somatotopic map formation in the zebrafish lateral line system. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(7):2058-2065.
Abstract
The zebrafish lateral line is a simple sensory system comprising a small number of neurons in addition to their sensory organs, the neuromasts. We have adopted this system as a model for single-cell level analyses of topographic map formation and examined when and how the lateral line topographic map is established. Single-neuron labeling demonstrated that somatotopic organization of the ganglion emerges by 54 hr postfertilization, but also that this initial map is not as accurate as that observed at 6 days postfertilization. During this initial stage, individual neurons exhibit extensively diverse behavior and morphologies. We identified leader neurons, the axons of which are the first to reach the tail, and later-appearing axons that contribute to the initial map. Our data suggest that lateral line neurons are heterogeneous from the beginning of lateral line development, and that some of them are intrinsically fate determined to contribute to the somatotopic map.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping