ZFIN ID: ZDB-PUB-100621-22
Single-cell analysis of somatotopic map formation in the zebrafish lateral line system
Sato, A., Koshida, S., and Takeda, H.
Date: 2010
Source: Developmental dynamics : an official publication of the American Association of Anatomists   239(7): 2058-2065 (Journal)
Registered Authors: Koshida, Sumito, Takeda, Hiroyuki
Keywords: lateral line, zebrafish, somatotopy, topographic map, single-cell, live imaging, neural circuit formation
MeSH Terms:
  • Animals
  • Lateral Line System/cytology
  • Lateral Line System/embryology*
  • Neurons/cytology
  • Zebrafish/embryology*
PubMed: 20549741 Full text @ Dev. Dyn.
The zebrafish lateral line is a simple sensory system comprising a small number of neurons in addition to their sensory organs, the neuromasts. We have adopted this system as a model for single-cell level analyses of topographic map formation and examined when and how the lateral line topographic map is established. Single-neuron labeling demonstrated that somatotopic organization of the ganglion emerges by 54 hr postfertilization, but also that this initial map is not as accurate as that observed at 6 days postfertilization. During this initial stage, individual neurons exhibit extensively diverse behavior and morphologies. We identified leader neurons, the axons of which are the first to reach the tail, and later-appearing axons that contribute to the initial map. Our data suggest that lateral line neurons are heterogeneous from the beginning of lateral line development, and that some of them are intrinsically fate determined to contribute to the somatotopic map.