PUBLICATION
Analyzing autophagy in zebrafish
- Authors
- He, C., and Klionsky, D.J.
- ID
- ZDB-PUB-100525-22
- Date
- 2010
- Source
- Autophagy 6(5): 642-644 (Journal)
- Registered Authors
- Keywords
- lysosome, protein degradation,k protein targeting, stress, vacuole
- MeSH Terms
-
- Amines/metabolism
- Animals
- Autophagy*
- Green Fluorescent Proteins/metabolism
- Microtubule-Associated Proteins/metabolism
- Recombinant Fusion Proteins/metabolism
- Staining and Labeling
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism
- PubMed
- 20495344 Full text @ Autophagy
Citation
He, C., and Klionsky, D.J. (2010) Analyzing autophagy in zebrafish. Autophagy. 6(5):642-644.
Abstract
The transparency, external development and simple drug administration of zebrafish embryos makes them a useful model for studying autophagy during embryonic development in vivo. Cloning of zebrafish lc3 and generation of a transgenic GFP-Lc3 fish line provide excellent tools to monitor autophagy in this organism.(1) This protocol discusses several convenient autophagy assays in zebrafish, including immunoblotting of Lc3 lipidation, microscopy imaging of GFP-Lc3 and lysosomal staining.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping