PUBLICATION
Silver exposure in developing Zebrafish (Danio rerio): Persistent effects on larval behavior and survival
- Authors
- Powers, C.M., Yen, J., Linney, E.A., Seidler, F.J., and Slotkin, T.A.
- ID
- ZDB-PUB-100202-18
- Date
- 2010
- Source
- Neurotoxicology and teratology 32(3): 391-397 (Journal)
- Registered Authors
- Linney, Elwood
- Keywords
- Developmental toxicity/neurotoxicity, Silver, Zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects*
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects
- Embryonic Development/drug effects*
- Environmental Pollutants/toxicity*
- Larva
- Metal Nanoparticles/toxicity*
- Silver/toxicity*
- Survival Analysis
- Zebrafish/embryology*
- Zebrafish/growth & development
- PubMed
- 20116428 Full text @ Neurotoxicol. Teratol.
Citation
Powers, C.M., Yen, J., Linney, E.A., Seidler, F.J., and Slotkin, T.A. (2010) Silver exposure in developing Zebrafish (Danio rerio): Persistent effects on larval behavior and survival. Neurotoxicology and teratology. 32(3):391-397.
Abstract
The increased use of silver nanoparticles in consumer and medical products has led to elevated human and environmental exposures. Silver nanoparticles act as antibacterial/antifungal agents by releasing Ag(+) and recent studies show that Ag(+) impairs neural cell replication and differentiation in culture, suggesting that in vivo exposures could compromise neurodevelopment. To determine whether Ag(+) impairs development in vivo, we examined the effects of exposure on survival, morphological, and behavioral parameters in zebrafish embryos and larvae. Methods: We exposed zebrafish from 0-5days post-fertilization to concentrations of Ag(+) ranging from 10nM to 100microM in order to assess effects on survival and early embryonic development. We then tested whether concentrations below the threshold for dysmorphology altered larval behavior and subsequent survival. Ag(+) concentrations>/=3microM significantly reduced embryonic survival, whereas 1microM delayed hatching with no effect on survival. Reducing the concentration to as low as 0.1microM delayed the inflation of the swim bladder without causing gross dysmorphology or affecting hatching. At this concentration, swimming activity was impaired, an effect that persisted past the point where swim bladder inflation became normal; in contrast, general motor function was unaffected. The early behavioral impairment was then predictive of subsequent decreases in survival. Ag(+) is a developmental toxicant within concentrations only slightly above allowable levels. At low concentrations, Ag(+) acts as a neurobehavioral toxicant even in the absence of dysmorphology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping