PUBLICATION
The iguana/DZIP1 protein is a novel component of the ciliogenic pathway essential for axonemal biogenesis
- Authors
- Tay, S.Y., Yu, X., Wong, K.N., Panse, P., Ng, C.P., and Roy, S.
- ID
- ZDB-PUB-091221-24
- Date
- 2010
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(2): 527-534 (Journal)
- Registered Authors
- Roy, Sudipto, Tay, Shang Yew
- Keywords
- cilia, axoneme, basal body, Hedgehog, DZIP1, zebrafish
- MeSH Terms
-
- Animals
- Axoneme/physiology*
- Carrier Proteins/metabolism*
- Cilia/physiology*
- Oncogene Proteins/metabolism
- Trans-Activators/metabolism
- Zebrafish
- PubMed
- 20014402 Full text @ Dev. Dyn.
Citation
Tay, S.Y., Yu, X., Wong, K.N., Panse, P., Ng, C.P., and Roy, S. (2010) The iguana/DZIP1 protein is a novel component of the ciliogenic pathway essential for axonemal biogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(2):527-534.
Abstract
Cilia play important roles in many developmental and physiological processes. However, the genetic and cell biological control of ciliogenesis remains poorly understood. Here, we show that the zebrafish iguana gene is required for differentiation of primary cilia. iguana encodes a zinc finger and coiled-coil containing protein, previously implicated in Hedgehog signaling. We now argue that aberrant Hedgehog activity in iguana -deficient zebrafish arises from their profound lack of primary cilia. By contrast, the requirement of iguana for motile cilia formation is less obligatory. In the absence of iguana function, basal bodies can migrate to the cell surface and appear to engage with the apical membrane. However, formation of ciliary pits and axonemal outgrowth is completely inhibited. Iguana localizes to the base of primary and motile cilia, in the immediate vicinity or closely associated with the basal bodies. These findings identify the Iguana protein as a novel and critical component of ciliogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping