ZFIN ID: ZDB-PUB-090616-33
Behavioral and neurotoxic effects of arsenic exposure in zebrafish (Danio rerio, Teleostei: Cyprinidae)
de Castro, M.R., Lima, J.V., Salomão, D., de Souza Valente, R., Dummer, N.S., de Aguiar, R.B., Dos Santos, L.C., Marins, L.F., Geracitano, L.A., Monserrat, J.M., and Barros, D.M.
Date: 2009
Source: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP   150(3): 337-342 (Journal)
Registered Authors: Marins, Luis Fernando
Keywords: Memory, Arsenic, Zebrafish, Behavioral, Inhibitory avoidance
MeSH Terms:
  • Animals
  • Arsenic/toxicity*
  • Avoidance Learning/drug effects*
  • Behavior, Animal/drug effects
  • Conditioning, Classical/drug effects*
  • Mecamylamine/pharmacology
  • Memory/drug effects
  • Nicotine/pharmacology
  • Oxidative Stress
  • Zebrafish
PubMed: 19501674 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
ABSTRACT
This study investigated the passive avoidance conditioning in zebrafish (Danio rerio). An instrument was developed for measuring escape responses triggered by a conditioned stimulus. This system allowed quantification of latency of crossing from a light to a dark zone. Zebrafish were trained to swim from an illuminated to a dark compartment, where they received a body shock (training session). The proposed methodology was efficient for evaluation of working, short, and long-term memory formation of an aquatic animal model. The possibility of employing memory measurements in toxicity tests, in order to obtain an ecologically meaningful biomarker response, was also analyzed. In this experiment, immediately after the training session, fish were exposed to three arsenic (As(V)) concentrations. After the test session, the brain was removed for biochemical analyses. A control group was kept in tap water. After exposure, animals were submitted to a one-trial inhibitory avoidance test for measurement of long-term memory (LTM). Results from behavioral and biochemical analysis showed that the three As(V) concentrations impaired LTM (p<0.05) and increased protein oxidation, which suggests an amnesic and pro-oxidant effect of As(V). Evaluation of behavior parameters in aquatic models is an important complement in studies concerning the environmental impact of chemical substances.
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