PUBLICATION
beta-Glucan administration enhances disease resistance and some innate immune responses in zebrafish (Danio rerio)
- Authors
- Rodríguez, I., Chamorro, R., Novoa, B., and Figueras, A.
- ID
- ZDB-PUB-090302-7
- Date
- 2009
- Source
- Fish & shellfish immunology 27(2): 369-373 (Journal)
- Registered Authors
- Figueras, Antonio, Novoa, Beatriz
- Keywords
- Zebrafish, β-glucan, Immune response, Disease resistance, Intraperitoneal injection, Aeromonas hydrophila
- MeSH Terms
-
- Adjuvants, Immunologic/pharmacology*
- Aeromonas hydrophila/physiology
- Animals
- Cytokines/blood
- Fish Diseases/immunology*
- Fish Diseases/mortality
- Gene Expression Regulation/drug effects
- Gram-Negative Bacterial Infections/immunology
- Gram-Negative Bacterial Infections/mortality
- Gram-Negative Bacterial Infections/veterinary*
- Immunity, Innate/drug effects*
- Kidney/cytology
- Survival Analysis
- Zebrafish/immunology*
- beta-Glucans/pharmacology*
- PubMed
- 19232393 Full text @ Fish Shellfish Immunol.
Citation
Rodríguez, I., Chamorro, R., Novoa, B., and Figueras, A. (2009) beta-Glucan administration enhances disease resistance and some innate immune responses in zebrafish (Danio rerio). Fish & shellfish immunology. 27(2):369-373.
Abstract
The present study was conducted to investigate the effect of beta-glucan (derived from Saccharomyces cerevisiae) on the immune response and its protection against an infection of the bacterial pathogen Aeromonas hydrophila in zebrafish (Danio rerio). Zebrafish received beta-glucan by intraperitoneal injection at three different concentrations (5, 2 and 0.5mgml(-1)) at 6, 4 and 2 days prior the challenge. On challenge day the control and beta-glucan pretreated zebrafish were intraperitoneally injected with A. hydrophila and mortality was recorded for 4 days. Intraperitoneal injection of 5mgml(-1) of beta-glucan significantly reduced the mortality. A single injection of 5mgml(-1) of beta-glucan 6 days before challenge also enhanced significantly the survival against the infection. The treatment with beta-glucan increased the myelomonocytic cell population in the kidney at 6h postchallenge with A. hydrophila. Moreover it enhanced the ability of kidney cells to kill A. hydrophila. beta-glucan did not affect the expression of TNFalpha or IL-1beta but seemed to modulate IFNgamma and chemokine expression in kidney.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping