|ZFIN ID: ZDB-PUB-090227-12|
A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation
Morley, R.H., Lachani, K., Keefe, D., Gilchrist, M.J., Flicek, P., Smith, J.C., and Wardle, F.C.
|Source:||Proceedings of the National Academy of Sciences of the United States of America 106(10): 3829-3834 (Journal)|
|Registered Authors:||Smith, Jim, Wardle, Fiona|
|Keywords:||brachyury, chromatin immunopreciptitation, microarray|
|PubMed:||19225104 Full text @ Proc. Natl. Acad. Sci. USA|
Morley, R.H., Lachani, K., Keefe, D., Gilchrist, M.J., Flicek, P., Smith, J.C., and Wardle, F.C. (2009) A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation. Proceedings of the National Academy of Sciences of the United States of America. 106(10):3829-3834.
ABSTRACTUsing chromatin immunoprecipitation combined with genomic microarrays we have identified targets of No tail (Ntl), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We show that Ntl regulates a downstream network of other transcription factors and identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. We show that the notochord-expressed gene floating head (flh) is a direct transcriptional target of Ntl and that a combination of TBSs in the flh upstream region are required for Ntl-directed expression. Using our genome-scale data we have assembled a preliminary gene regulatory network that begins to describe mesoderm formation and patterning in the early zebrafish embryo.