ZFIN ID: ZDB-PUB-090227-12
A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation
Morley, R.H., Lachani, K., Keefe, D., Gilchrist, M.J., Flicek, P., Smith, J.C., and Wardle, F.C.
Date: 2009
Source: Proceedings of the National Academy of Sciences of the United States of America   106(10): 3829-3834 (Journal)
Registered Authors: Smith, Jim, Wardle, Fiona
Keywords: brachyury, chromatin immunopreciptitation, microarray
Microarrays: GEO:GSE12331
MeSH Terms:
  • Animals
  • Base Sequence
  • Binding Sites
  • Body Patterning/genetics
  • Cell Lineage
  • Conserved Sequence
  • Fetal Proteins/genetics
  • Fetal Proteins/metabolism*
  • Gastrulation/genetics
  • Gene Expression Regulation, Developmental
  • Gene Regulatory Networks*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Mesoderm/embryology*
  • Molecular Sequence Data
  • Muscles/cytology
  • Protein Binding
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/metabolism*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transcription, Genetic
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 19225104 Full text @ Proc. Natl. Acad. Sci. USA
Using chromatin immunoprecipitation combined with genomic microarrays we have identified targets of No tail (Ntl), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We show that Ntl regulates a downstream network of other transcription factors and identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. We show that the notochord-expressed gene floating head (flh) is a direct transcriptional target of Ntl and that a combination of TBSs in the flh upstream region are required for Ntl-directed expression. Using our genome-scale data we have assembled a preliminary gene regulatory network that begins to describe mesoderm formation and patterning in the early zebrafish embryo.