PUBLICATION
Cloning and sequence analysis of cDNA for the proteasome activator PA28-beta subunit of flounder (Paralichthys olivaceus)
- Authors
- Kim, D.H., Lee, S.M., Hong, B.Y., Kim, Y.T., and Choi, T.J.
- ID
- ZDB-PUB-090225-4
- Date
- 2003
- Source
- Molecular immunology 40(9): 611-616 (Journal)
- Registered Authors
- Keywords
- Flounder, Proteasome activator, PA28-β, PSME2
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Complementary
- Flounder/genetics*
- Molecular Sequence Data
- Muscle Proteins/genetics*
- Phylogeny
- Protein Subunits/genetics
- Proteins/genetics*
- Sequence Analysis, DNA
- Zebrafish Proteins*
- PubMed
- 14597164 Full text @ Mol. Immunol.
Citation
Kim, D.H., Lee, S.M., Hong, B.Y., Kim, Y.T., and Choi, T.J. (2003) Cloning and sequence analysis of cDNA for the proteasome activator PA28-beta subunit of flounder (Paralichthys olivaceus). Molecular immunology. 40(9):611-616.
Abstract
Proteasome is a large multisubunit complex involved in intracellular proteolysis in antigen processing for loading MHC class I molecules. Two activators PA28-alpha and PA28-beta, which are induced by interferon-gamma (IFN-gamma), activate this latent enzyme complex. Genes encoding these activators, PSME1 and PSME2, respectively, have been characterized from various mammalian but only from zebrafish among piscine. We have cloned a PSME2 gene homologue from a leukocyte cDNA library of flounder, a marine fish. The flounder PSME2 gene (fPSME2) encompasses 1063 nucleotides and encodes a polypeptide of 242 amino acids (aa), with a deduced molecular weight of 27.2 kDa. The deduced protein has 82% sequence similarity to that of zebrafish and 73-74% sequence similarity to that of various mammalians and shows higher level sequence homology in the C-terminal region. There was a PA28-beta protein subunit-specific insert located at the corresponding to the KEKE motif of PA28-alpha protein. A phylogenetic tree derived using deduced amino acid sequences showed a diversion of piscine PSME2 from mammalian counterpart after diversion of PSME1 and PSME2 from a common ancestral gene. Northern blot analysis revealed a higher level expression of fPSME2 gene in kidney, spleen and muscle tissues of bacterial lipopolysaccharide (LPS) stimulated flounder than those from non-induced flounder.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping