|ZFIN ID: ZDB-PUB-090123-9|
Your Input Welcome
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.
Connexin43 regulates joint location in zebrafish fins
Sims Jr, K., Eble, D.M., and Iovine, M.K.
|Source:||Developmental Biology 327(2): 410-418 (Journal)|
|Registered Authors:||Iovine, M. Kathryn|
|Keywords:||Joint morphogenesis, Fin growth, Zebrafish, Gap junctions, Cx43, Short fin, Another long fin|
|PubMed:||19150347 Full text @ Dev. Biol.|
Sims Jr, K., Eble, D.M., and Iovine, M.K. (2009) Connexin43 regulates joint location in zebrafish fins. Developmental Biology. 327(2):410-418.
ABSTRACTJoints are essential for skeletal form and function, yet their development remains poorly understood. In zebrafish fins, joints form between the bony fin ray segments providing essentially unlimited opportunities to evaluate joint morphogenesis. Mutations in cx43 cause the short segment phenotype of short fin (sof(b123)) mutants, suggesting that direct cell-cell communication may regulate joint location. Interestingly, increased cx43 expression in the another long fin (alf(dty86)) mutant appears to cause joint failure typical of that mutant. Indeed, knockdown of cx43 in alf(dty86) mutant fins rescues joint formation. Together, these data reveal a correlation between the level of Cx43 expression in the fin ray mesenchyme and the location of joints. Cx43 was also observed laterally in cells associated with developing joints. Confocal microscopy revealed that the Cx43 protein initially surrounds the membranes of ZNS5-positive joint cells, but at later stages becomes polarized toward the underlying Cx43-positive mesenchymal cells. One possibility is that communication between the Cx43-positive mesenchyme and the overlying ZNS5-positive cells regulates joint location, and upregulation of Cx43 in joint-forming cells contributes to joint morphogenesis.