PUBLICATION
G2 acquisition by transcription-independent mechanism at the zebrafish midblastula transition
- Authors
- Dalle Nogare, D.E., Pauerstein, P.T., and Lane, M.E.
- ID
- ZDB-PUB-081217-24
- Date
- 2009
- Source
- Developmental Biology 326(1): 131-142 (Journal)
- Registered Authors
- Lane, Mary Ellen
- Keywords
- none
- MeSH Terms
-
- Animals
- Blastula/physiology*
- CDC2 Protein Kinase/physiology
- Cell Cycle/physiology
- Cyclin-Dependent Kinase 2/physiology
- G2 Phase/physiology*
- Transcriptional Activation/physiology
- Zebrafish/embryology*
- Zebrafish Proteins/physiology*
- cdc25 Phosphatases/physiology
- PubMed
- 19063878 Full text @ Dev. Biol.
Citation
Dalle Nogare, D.E., Pauerstein, P.T., and Lane, M.E. (2009) G2 acquisition by transcription-independent mechanism at the zebrafish midblastula transition. Developmental Biology. 326(1):131-142.
Abstract
Following fertilization of many animal embryos, rapid synchronous cleavage divisions give way to longer, asynchronous cell cycles at the midblastula transition (MBT). The cell cycle changes at the MBT, including the addition of gap phases and checkpoint controls, are accompanied by activation of the zygotic genome and the onset of cell motility. Whereas the biochemical changes accompanying the MBT in the vertebrate embryo have been extensively documented, the cellular events are not well understood. We show that cell cycle remodeling during the zebrafish MBT includes the transcription-independent acquisition of a G2 phase that is essential for preventing entry into mitosis before S-phase completion in cycles 11-13. We provide evidence from high-resolution imaging that inhibition of Cdc25a and Cdk1 activity, but not Cdk2 activity, is essential for cell cycle lengthening and asynchrony between cycles 9 and 12. We demonstrate that lengthening is not required for initiation of zygotic transcription. Our results are consistent with findings from Drosophila and Xenopus that indicate the central importance of G2 addition in checkpoint establishment, and point to similar mechanisms governing the MBT in diverse species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping