ZFIN ID: ZDB-PUB-081105-13
Using retroviruses as a mutagenesis tool to explore the zebrafish genome
Jao, L.E., Maddison, L., Chen, W., and Burgess, S.M.
Date: 2008
Source: Briefings in functional genomics & proteomics 7(6): 427-443 (Review)
Registered Authors: Burgess, Shawn, Chen, Wenbiao
Keywords: genetics, moloney murine leukemia virus, enhancer traps, gene traps, linker-mediated PCR, zebrafish
MeSH Terms: Animals; Genome*; Models, Biological*; Mutagenesis*; Retroviridae/genetics* (all 6) expand
PubMed: 18977782 Full text @ Brief. Funct. Genomic. Proteomic.
ABSTRACT
We review different uses of the retroviral mutagenesis technology as the tool to manipulate the zebrafish genome. In addition to serving as a mutagen in a phenotype-driven forward mutagenesis screen as it was originally adapted for, retroviral insertional mutagenesis can also be exploited in reverse genetic approaches, delivering enhancer- and gene-trap vectors for the purpose of examining gene expression patterns and mutagenesis, making sensitized mutants amenable for chemical and genetic modifier screens, and producing gain-of-function mutations by epigenetically overexpressing the retroviral-inserted genes. From a technology point of view, we also summarize the recent advances in the high-throughput cloning of retroviral integration sites, a pivotal step toward identifying mutations. Lastly, we point to some potential directions that retroviral mutagenesis might take from the lessons of studying other model organisms.
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