PUBLICATION

Molecular cloning and expression analysis of the STAT1 gene from olive flounder, Paralichthys olivaceus

Authors
Park, E.M., Kang, J.H., Seo, J.S., Kim, G., Chung, J., and Choi, T.J.
ID
ZDB-PUB-080630-9
Date
2008
Source
BMC immunology   9(1): 31 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular*
  • Flounder/genetics*
  • Flounder/growth & development
  • Flounder/metabolism
  • Gene Expression
  • Molecular Sequence Data
  • Phylogeny
  • Protein Structure, Tertiary
  • STAT1 Transcription Factor/chemistry
  • STAT1 Transcription Factor/genetics*
  • STAT1 Transcription Factor/metabolism
  • Sequence Alignment
  • Signal Transduction
  • src Homology Domains
PubMed
18578892 Full text @ BMC Immunol.
Abstract
BACKGROUND: Signal transducer and activator of transcription 1 (STAT1) is a critical component of interferon (IFN)-alpha/beta and IFN-gamma signaling. Although seven isoforms of STAT proteins have been reported from mammals, limited information is available for the STAT genes in fish. We isolated complementary DNA with high homology to mammalian STAT1 from the olive flounder, Paralichthys olivaceus. RESULTS: A DNA fragment containing the conserved SH2 domain was amplified by RT-PCR using degenerate primers designed based on the highly conserved sequences in the SH2 domains of the zebrafish and mammalian STAT1. The complete cDNA sequence was obtained by 5a and 3a RACE. The flounder STAT1 transcript consisted of 2,909 bp that encoded a polypeptide of 749 amino acids. The overall homology between flounder STAT1 and other STATs was very high, with the highest amino acid sequence identity to snakehead (89%). Phylogenetic analyses reveal that flounder STAT1 is in the same monophyletic group with snakehead STAT1. RT-PCR and in situ hybridization revealed that STAT1 was expressed in almost all examined organs and tissues, with high expression in the spleen, kidney, intestine and gill. The accumulation of STAT1 mRNA in different developmental stages, as determined by RT-PCR, increased with development. CONCLUSION: Recent cloning of various cytokine genes and the STAT1 gene of olive flounder here suggest that fish also use the highly specialized JAK-STAT pathway for cytokine signaling. Identification of other STAT genes will elucidate in detail the signal transduction system in this fish.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping