ZFIN ID: ZDB-PUB-080331-15
Mechanism unknown: prostaglandin E2 may improve HSC therapies
White, R.M., Sessa, A., Burke, C., Bowman, T., LeBlanc, J., Ceol, C., Bourque, C., Dovey, M., Goessling, W., Burns, C.E., and Zon, L.I.
Date: 2008
Source: Cell Stem Cell 2(2): 183-189 (Journal)
Registered Authors: Bourque, Caitlin, Bowman, Teresa, Burke, Christopher, Burns (Erter), Caroline, Ceol, Craig, Dovey, Michael, Goessling, Wolfram, LeBlanc, Jocelyn, Sessa, Anna, White, Richard M., Zon, Leonard I.
Keywords: STEMCELL, DEVBIO
MeSH Terms: Albinism/genetics; Animals; Flow Cytometry; Light; Models, Animal* (all 10) expand
PubMed: 18371439 Full text @ Cell Stem Cell
ABSTRACT
The zebrafish is a useful model for understanding normal and cancer stem cells, but analysis has been limited to embryogenesis due to the opacity of the adult fish. To address this, we have created a transparent adult zebrafish in which we transplanted either hematopoietic stem/progenitor cells or tumor cells. In a hematopoiesis radiation recovery assay, transplantation of GFP-labeled marrow cells allowed for striking in vivo visual assessment of engraftment from 2 hr-5 weeks posttransplant. Using FACS analysis, both transparent and wild-type fish had equal engraftment, but this could only be visualized in the transparent recipient. In a tumor engraftment model, transplantation of RAS-melanoma cells allowed for visualization of tumor engraftment, proliferation, and distant metastases in as little as 5 days, which is not seen in wild-type recipients until 3 to 4 weeks. This transparent adult zebrafish serves as the ideal combination of both sensitivity and resolution for in vivo stem cell analyses.
ADDITIONAL INFORMATIONNo data available