PUBLICATION
Survival, excitability, and transfection of retinal neurons in an organotypic culture of mature zebrafish retina
- Authors
- Kustermann, S., Schmid, S., Biehlmaier, O., and Kohler, K.
- ID
- ZDB-PUB-080326-7
- Date
- 2008
- Source
- Cell and tissue research 332(2): 195-209 (Journal)
- Registered Authors
- Biehlmaier, Oliver
- Keywords
- Neurogenesis, Adult retina, Regeneration, In vitro system, Visual system, Zebrafish, Danio rerio (Teleostet)
- MeSH Terms
-
- Animals
- Apoptosis
- Cell Proliferation
- Electroporation
- Neuroglia/cytology
- Neuroglia/physiology
- Neurons/cytology
- Neurons/physiology*
- Organ Culture Techniques*
- Patch-Clamp Techniques
- Retina/cytology*
- Retina/growth & development
- Retina/physiology*
- Retinal Ganglion Cells/cytology
- Retinal Ganglion Cells/physiology*
- Transfection
- Zebrafish
- PubMed
- 18335243 Full text @ Cell Tissue Res.
Citation
Kustermann, S., Schmid, S., Biehlmaier, O., and Kohler, K. (2008) Survival, excitability, and transfection of retinal neurons in an organotypic culture of mature zebrafish retina. Cell and tissue research. 332(2):195-209.
Abstract
Over the last 20 years, the zebrafish has become an important model organism for research on retinal function and development. Many retinal diseases do not become apparent until the later stages of life. This means that it is important to be able to analyze (gene) function in the mature retina. To meet this need, we have established an organotypic culture system of mature wild-type zebrafish retinas in order to observe changes in retinal morphology. Furthermore, cell survival during culture has been monitored by determining apoptosis in the tissue. The viability and excitability of ganglion cells have been tested at various time points in vitro by patch-clamp recordings, and retinal functionality has been assessed by measuring light-triggered potentials at the ganglion cell site. Sinin the tissue. The viability and excitability of ganglion cells have been tested at various time points in vitro by patch-clamp recordings, and retce neurogenesis is persistent in adult zebrafish retinas, we have also monitored proliferating cells during culture by tracking their bromodeoxyuridine uptake. Reverse genetic approaches for probing the function of adult zebrafish retinas are not yet available. We have therefore established a rapid and convenient protocol for delivering plasmid DNA or oligonucleotides by electroporation to the retinal tissue in vitro. The organotypic culture of adult zebrafish retinas presented here provides a reproducible and convenient method for investigating the function of drugs and genes in the retina under well-defined conditions in vitro.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping