ZFIN ID: ZDB-PUB-080326-28
Fgf-dependent depletion of microRNA-133 promotes appendage regeneration in zebrafish
Yin, V.P., Thomson, J.M., Thummel, R., Hyde, D.R., Hammond, S.M., and Poss, K.D.
Date: 2008
Source: Genes and Development 22(6): 728-733 (Journal)
Registered Authors: Hyde, David R., Poss, Kenneth D., Thummel, Ryan, Yin, Voot
Keywords: Zebrafish, fin, regeneration, miR-133, Fgf, Mps1
MeSH Terms:
  • Animals
  • Blotting, Northern
  • Cell Proliferation
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/physiology
  • Extremities/physiology*
  • Fibroblast Growth Factors/metabolism*
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Developmental*
  • MicroRNAs/physiology*
  • Protein-Serine-Threonine Kinases/metabolism
  • Protein-Tyrosine Kinases/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Receptors, Fibroblast Growth Factor/metabolism
  • Regeneration/physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/physiology*
  • Zebrafish Proteins/metabolism
PubMed: 18347091 Full text @ Genes Dev.
ABSTRACT
Appendage regeneration is defined by rapid changes in gene expression that achieve dramatic developmental effects, suggesting involvement of microRNAs (miRNAs). Here, we find dynamic regulation of many miRNAs during zebrafish fin regeneration. In particular, miR-133 levels are high in uninjured fins but low during regeneration. When regeneration was blocked by Fibroblast growth factor (Fgf) receptor inhibition, high miR-133 levels were quickly restored. Experimentally increasing amounts of miR-133 attenuated fin regeneration. Conversely, miR-133 antagonism during Fgf receptor inhibition accelerated regeneration through increased proliferation within the regeneration blastema. The Mps1 kinase, an established positive regulator of blastemal proliferation, is an in vivo target of miR-133. Our findings identify miRNA depletion as a new regulatory mechaneneration through increased proliferation within the regeneration blastema. The Mps1 kinase, an established positive regulator of blastemal prolifeism for complex tissue regeneration.
ADDITIONAL INFORMATIONNo data available