PUBLICATION
Egfl7 knockdown causes defects in the extension and junctional arrangements of endothelial cells during zebrafish vasculogenesis
- Authors
- De Mazière, A., Parker, L., Van Dijk, S., Ye, W., and Klumperman, J.
- ID
- ZDB-PUB-080226-10
- Date
- 2008
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 237(3): 580-591 (Journal)
- Registered Authors
- Parker, Leon
- Keywords
- zebrafish, EGFL7, angiogenesis, vasculogenesis, hematopoiesis, endothelial cells, cell junctions, desmosome, polarity, transferrin receptor
- MeSH Terms
-
- Animals
- Blood Vessels/embryology*
- Cell Polarity
- Embryo, Nonmammalian
- Endothelial Cells/physiology
- Endothelial Cells/ultrastructure*
- Endothelium, Vascular/ultrastructure
- Erythroblasts/ultrastructure
- Intercellular Junctions/ultrastructure
- Microscopy, Electron, Transmission
- Neovascularization, Physiologic*
- Zebrafish/embryology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 18224713 Full text @ Dev. Dyn.
Citation
De Mazière, A., Parker, L., Van Dijk, S., Ye, W., and Klumperman, J. (2008) Egfl7 knockdown causes defects in the extension and junctional arrangements of endothelial cells during zebrafish vasculogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(3):580-591.
Abstract
The endothelial cell (EC) -specific secreted protein EGFL7 is important for tubulogenesis in newly forming blood vessels. We studied its role in vascular tube formation by a quantitative ultrastructural analysis of Egfl7-knockdown zebrafish embryos. At 24 hours postfertilization, the endothelia of dorsal aorta (DA) and posterior cardinal vein (PCV) were correctly anchored to the hypochord and endoderm, respectively, but failed to expand into the vascular area. This resulted in vessels with reduced or split lumen and open sheets of ECs. Concomitantly, the organization of hematopoietic cells-identified by the presence of previously undescribed membrane tubules-between DA and PCV, and within the vessels, was severely disturbed. Strikingly, ectopic cell junctions occurred across the obstructed vessel lumen, on the luminal EC surfaces, which in control conditions never display junctions of any kind. These data suggest that Egfl7 provides ECs with a cue for their extension into the vascular area and in establishing EC cell polarity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping