PUBLICATION
The Lengthening of a Giant Protein: When, How, and Why?
- Authors
- Meiniel, O., Meiniel, R., Lalloué, F., Didier, R., Jauberteau, M.O., Meiniel, A., and Petit, D.
- ID
- ZDB-PUB-071210-10
- Date
- 2008
- Source
- Journal of molecular evolution 66(1): 1-10 (Journal)
- Registered Authors
- Keywords
- SCO-spondin, Central nervous system, Thrombospondin type 1 repeat, SCO-spondin repeat, Deuterostomia
- MeSH Terms
-
- Amino Acid Motifs
- Amino Acid Sequence
- Animals
- Cell Adhesion Molecules, Neuronal/chemistry*
- Cell Adhesion Molecules, Neuronal/classification*
- Cell Adhesion Molecules, Neuronal/genetics
- Consensus Sequence
- Conserved Sequence
- Evolution, Molecular*
- Phylogeny
- Protein Structure, Tertiary
- Repetitive Sequences, Amino Acid
- Zebrafish/embryology
- Zebrafish/metabolism
- Zebrafish Proteins/chemistry*
- Zebrafish Proteins/classification*
- Zebrafish Proteins/genetics
- PubMed
- 18046595 Full text @ J. Mol. Evol.
Citation
Meiniel, O., Meiniel, R., Lalloué, F., Didier, R., Jauberteau, M.O., Meiniel, A., and Petit, D. (2008) The Lengthening of a Giant Protein: When, How, and Why?. Journal of molecular evolution. 66(1):1-10.
Abstract
Subcommissural organ (SCO)-spondin is a giant glycoprotein of more than 5000 amino acids found in Vertebrata, expressed in the central nervous system and constitutive of Reissner's fiber. For the first time, in situ hybridization performed on zebrafish (Danio rerio) embryos shows that the gene encoding this protein is expressed transitionally in the floor plate, the ventral midline of the neural tube, and later in the diencephalic third ventricle roof, the SCO. The modular organization of the protein in Echinodermata (Strongylocentrotus purpuratus), Urochordata (Ciona savignyi and C. intestinalis), and Vertebrata (Teleostei, Amphibia, Aves and Mammalia) is also described. As the thrombospondin type 1 repeat motifs represent an increasingly large part of the protein during Deuterostomia evolution, the duplication mechanisms leading to this complex organization are examined. The functional significance of the particularly well-preserved arrangement of the series of SCO-spondin repeat motifs and thombospondin type 1 repeats is discussed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping