ZFIN ID: ZDB-PUB-071001-12
Identification and characterization of zebrafish semaphorin 6D
Kimura, M., Taniguchi, M., Mikami, Y., Masuda, T., Yoshida, T., Mishina, M., and Shimizu, T.
Date: 2007
Source: Biochemical and Biophysical Research Communications   363(3): 762-768 (Journal)
Registered Authors: Mikami, Yoshinori, Mishina, Masayoshi, Yoshida, Tomoyuki
Keywords: Semaphorin, Plexin, Axon guidance, Brain, Lens, Rhombomere, Repellent
MeSH Terms:
  • Animals
  • Cell Line
  • Chick Embryo
  • Cloning, Molecular
  • DNA, Complementary/chemistry
  • DNA, Complementary/genetics
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Ganglia, Spinal/embryology
  • Ganglia, Spinal/metabolism
  • Gene Expression Profiling*
  • Gene Expression Regulation, Developmental
  • Humans
  • In Situ Hybridization
  • Molecular Sequence Data
  • Protein Binding
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism
  • Semaphorins/genetics*
  • Semaphorins/metabolism
  • Sequence Analysis, DNA
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 17897628 Full text @ Biochem. Biophys. Res. Commun.
The semaphorin gene family contains a large number of secreted and transmembrane proteins; some function as repulsive and attractive cues of axon guidance during development. Here, we report cloning and characterization of zebrafish transmembrane semaphorin gene, semaphorin 6D (sema6D). Sema6D is expressed predominantly in the nervous system during embryogenesis, as determined by in situ hybridization. We also found that Sema6D binds Plexin-A1 in vitro, but not other Plexins. It induces the repulsion of dorsal root ganglion axons, but not sympathetic axons. Consequently, Sema6D might use Plexin-A1 as a receptor to repel specific types of axons during development.