PUBLICATION
Innate immune gene expression in individual zebrafish after Listonella anguillarum inoculation
- Authors
- Rojo, I., de Ilárduya, O.M., Estonba, A., and Pardo, M.A.
- ID
- ZDB-PUB-070907-37
- Date
- 2007
- Source
- Fish & shellfish immunology 23(6): 1285-1293 (Journal)
- Registered Authors
- Keywords
- Danio rerio, Cytokines, il1b, cebpb, Immunity, Real-time RT-PCR, Variability
- MeSH Terms
-
- Animals
- DNA Primers/chemistry
- Fish Diseases/immunology*
- Fish Diseases/microbiology
- Gene Expression Regulation/immunology*
- Gram-Negative Bacterial Infections/immunology
- Gram-Negative Bacterial Infections/microbiology
- Gram-Negative Bacterial Infections/veterinary*
- Immunity, Innate/immunology*
- Listonella/immunology*
- Reverse Transcriptase Polymerase Chain Reaction/veterinary
- Time Factors
- Zebrafish/immunology*
- PubMed
- 17804254 Full text @ Fish Shellfish Immunol.
Citation
Rojo, I., de Ilárduya, O.M., Estonba, A., and Pardo, M.A. (2007) Innate immune gene expression in individual zebrafish after Listonella anguillarum inoculation. Fish & shellfish immunology. 23(6):1285-1293.
Abstract
Zebrafish were intraperitoneally injected with 10(6)CFU (LD(50)) Listonella anguillarum. Three inoculated and control fish were collected at 1, 2, 4, 6 and 22h post infection (hpi) and the expression of genes related to the immune response (il1b, cebpb, tfa, mpx, tnfa, nitr9, tlr22, hsc70, cp, mrlp1, c3b and lyz) in each fish was monitored by means of real-time RT-PCR. A similar experiment was performed considering an intermediate time point at 15hpi. Different relative levels of expression were found among genes. Also, wide interindividual variation in gene expression for most genes was detected among fish, inoculated or not. A steady increase of expression starting from the initial stages of the interaction was found for interleukin-1beta. An initial increase in levels of gene expression was found for the genes coding for the CCAAT/Enhancer Binding Protein subunit beta and the Novel Immune-Type Receptor 9, although their levels decreased later on and were indistinguishable from the controls at 22hpi. Finally, some genes (Transferrin, Myeloid-specific Peroxidase and Tumour Necrosis Factor alpha) were upregulated at 22hpi. Taken together, our results show an induction in gene expression of genes involved in the inflammatory and immune response upon L. anguillarum infection but also reveal the existence of a wide variation in the levels of expression of the studied genes in the zebrafish population.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping