PUBLICATION
Molecular cloning and expression analysis of T-bet in ginbuna crucian carp (Carassius auratus langsdorfii)
- Authors
- Takizawa, F., Araki, K., Kobayashi, I., Moritomo, T., Ototake, M., and Nakanishi, T.
- ID
- ZDB-PUB-070726-7
- Date
- 2008
- Source
- Molecular immunology 45(1): 127-136 (Journal)
- Registered Authors
- Keywords
- T-bet, Transcription factor, T-cell, Teleost, Ginbuna crucian carp, Lymphocyte
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Chromosomes/genetics
- Cloning, Molecular
- Gene Expression Profiling
- Gene Expression Regulation
- Goldfish/genetics*
- Humans
- Molecular Sequence Data
- Phylogeny
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Synteny/genetics
- T-Box Domain Proteins/chemistry
- T-Box Domain Proteins/genetics*
- T-Box Domain Proteins/metabolism
- Tissue Distribution
- PubMed
- 17624433 Full text @ Mol. Immunol.
Citation
Takizawa, F., Araki, K., Kobayashi, I., Moritomo, T., Ototake, M., and Nakanishi, T. (2008) Molecular cloning and expression analysis of T-bet in ginbuna crucian carp (Carassius auratus langsdorfii). Molecular immunology. 45(1):127-136.
Abstract
In the adaptive immune system of mammals, naive helper T (Th) cells differentiate into Th1 or Th2 cells. The T-box expressed in T cells (T-bet) is a member of a family of T-box transcription factors that regulates the expression of IFN-gamma and plays a crucial role in Th1 cell differentiation and cell-mediated immunity. We cloned and sequenced T-bet cDNA for the first time from non-mammalian species, ginbuna crucian carp. Ginbuna T-bet was composed of 608 predicted amino acids and showed 41.5% identity with human T-bet (Tbx21), and human and ginbuna T-bet share 77.3% identity in their T-box regions. Comparative genomic analysis showed conserved synteny in these regions between zebrafish, fugu, medaka and human T-bet. Phylogenetic analysis indicated that ginbuna T-bet is closely related to that of mouse and human. In unstimulated fish, ginbuna T-bet mRNA was strongly expressed in peripheral blood leukocytes (PBL), head kidney (HK) and spleen. RT-PCR analysis in kidney cells sorted by FACS revealed that T-bet was strongly expressed in surface-IgM-negative lymphocytes in comparison to IgM-positive lymphocytes. These results suggest that ginbuna T-bet is involved in the immune system, especially in T-cell function, and is an important tool to analyze teleost cell-mediated immunity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping