PUBLICATION

Targeting neural circuitry in zebrafish using GAL4 enhancer trapping

Authors
Scott, E.K., Mason, L., Arrenberg, A.B., Ziv, L., Gosse, N.J., Xiao, T., Chi, N.C., Asakawa, K., Kawakami, K., and Baier, H.
ID
ZDB-PUB-070330-18
Date
2007
Source
Nature Methods   4(4): 323-326 (Journal)
Registered Authors
Arrenberg, Aristides, Baier, Herwig, Gosse, Nathan, Kawakami, Koichi, Mason, Lindsay, Scott, Ethan, Xiao, Tong, Ziv, Limor
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Brain*/cytology
  • Brain*/embryology
  • Brain*/metabolism
  • DNA-Binding Proteins
  • Enhancer Elements, Genetic*
  • Gene Expression Regulation, Developmental*
  • Humans
  • Neurons*/metabolism
  • Pilot Projects
  • Saccharomyces cerevisiae Proteins/biosynthesis
  • Saccharomyces cerevisiae Proteins/genetics*
  • Transcription Factors/biosynthesis
  • Transcription Factors/genetics*
  • Transgenes
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed
17369834 Full text @ Nat. Methods
Abstract
We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes