ZFIN ID: ZDB-PUB-070212-26
Knockdown of V-ATPase subunit A ({alpha}tp6v1{alpha}) impairs acid secretion and ion balance in zebrafish Danio rerio
Horng, J.L., Lin, L.Y., Huang, C.J., Katoh, F., Kaneko, T., and Hwang, P.P.
Date: 2007
Source: American journal of physiology. Regulatory, integrative and comparative physiology   292(5): R2068-2076 (Journal)
Registered Authors: Horng, Jiun-Lin, Huang, Chang-Jen, Hwang, Pung Pung
Keywords: H+-ATPase, HR cell, morpholino-knockdown, Na+, Ca2+
MeSH Terms:
  • Acids/metabolism*
  • Animals
  • Embryo, Nonmammalian/metabolism*
  • Fresh Water
  • Gene Expression Regulation, Enzymologic
  • Mutation
  • Protein Subunits/deficiency
  • Protein Subunits/genetics
  • Protein Subunits/metabolism
  • Proton-Translocating ATPases/chemistry
  • Proton-Translocating ATPases/deficiency*
  • Proton-Translocating ATPases/genetics
  • Proton-Translocating ATPases/metabolism
  • Protons
  • Vacuolar Proton-Translocating ATPases/deficiency
  • Vacuolar Proton-Translocating ATPases/genetics*
  • Vacuolar Proton-Translocating ATPases/metabolism*
  • Water-Electrolyte Balance*
  • Yolk Sac/metabolism
  • Zebrafish/metabolism*
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
PubMed: 17272665 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
In the skin of zebrafish embryo, the vacuolar H(+)-ATPase (V-ATPase, H(+) pump) distributed mainly in the apical membrane of H(+)-pump rich cells, which pump internal acid out of the embryo and function similar to acid-secreting intercalated cells in mammalian kidney. In addition to acid excretion, the electrogenic H(+) efflux via the H(+)-ATPases in the gill apical membrane of freshwater fish was proposed to act as a driving force for Na(+) entry through the apical Na(+) channels. However, convincing molecular physiological evidence in vivo for this model is still lacking. In this study, we used morpholino-modified antisense oligo-nucleotides to knockdown the gene product of H(+)-ATPase subunit A (alphatp6v1alpha) and examined the phenotype of the mutants. The H(+)-ATP knockdown embryos revealed several abnormalities, including suppression of acid-secretion from skin, growth retardation, trunk deformation, and loss of internal Ca(2+) and Na(+). This finding reveals the critical role of H(+)-ATPase in embryonic acid-secretion and ion balance as well. Key words: H+-ATPase, morpholino-knockdown, acid/base regulation, Na+, Ca2+