ZFIN ID: ZDB-PUB-061031-10
Localization of anosmin-1a and anosmin-1b in the inner ear and neuromasts of zebrafish
Ernest, S., Guadagnini, S., Prevost, M.C., and Soussi-Yanicostas, N.
Date: 2007
Source: Gene expression patterns : GEP   7(3): 274-281 (Journal)
Registered Authors: Ernest, Sylvain, Soussi-Yanicostas, Nadia
Keywords: Zebrafish, Kallmann syndrome, Kal1a, Kal1b, Anosmin-1a, Anosmin-1b, Neuromast, Inner ear, Hair cells, Kinocilia, Stereocilia, Lateral line, Crista, Anosmia, Hypogonadism, Anti-anosmin-1a, Anti-anosmin-1b, Scanning electron microscopy
MeSH Terms:
  • Animals
  • Ear, Inner/anatomy & histology
  • Ear, Inner/chemistry*
  • Ear, Inner/metabolism
  • Extracellular Matrix Proteins/analysis*
  • Extracellular Matrix Proteins/genetics
  • Fluorescent Antibody Technique
  • Hair Cells, Auditory/chemistry*
  • Hair Cells, Auditory/metabolism
  • Hair Cells, Auditory/ultrastructure
  • Immunohistochemistry
  • Lateral Line System/chemistry*
  • Lateral Line System/cytology
  • Lateral Line System/metabolism
  • Microscopy, Confocal
  • Microscopy, Electron, Scanning
  • Microscopy, Immunoelectron
  • Nerve Tissue Proteins/analysis*
  • Nerve Tissue Proteins/genetics
  • Sensory Receptor Cells/chemistry*
  • Sensory Receptor Cells/metabolism
  • Zebrafish/anatomy & histology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed: 17064968 Full text @ Gene Expr. Patterns
Anosmin-1, encoded by the KAL-1 gene, is the protein defective in the X-linked form of Kallmann syndrome. This human developmental disorder is characterized by defects in cell migration and axon target selection. Anosmin-1 is an extracellular matrix protein that plays a role, in vitro, in processes such as cell adhesion, neurite outgrowth, axon guidance, and axon branching. The zebrafish possesses two orthologues of the KAL-1 gene: kal1a and kal1b, which encode anosmin-1a and anosmin-1b, respectively. Previous in situ hybridization studies have shown that kal1a and kal1b mRNAs are expressed in undetermined cells of the inner ear but not in neuromast cells. Using specific antibodies against anosmin-1a and anosmin-1b, we report here that both proteins are expressed in sensory hair cells of the inner ear cristae ampullaris and the lateral line neuromasts. Accumulation of these proteins was observed mainly at the level of the hair bundle and also at the cell membrane. In neuromast hair cells, immunogold scanning electronmicroscopy demonstrated that anosmin-1a and anosmin-1b were present at the surface of the stereociliary bundle. In addition, anosmin-1a, but not anosmin-1b, was detected on the track of the ampullary nerve. This is the first report of anosmin-1 expression in sensory hair cells of the inner ear and lateral line, and along the ampullary nerve track.