PUBLICATION
Ribonucleases and angiogenins from fish
- Authors
- Pizzo, E., Buonanno, P., Di Maro, A., Ponticelli, S., De Falco, S., Quarto, N., Cubellis, M.V., and D'Alessio, G.
- ID
- ZDB-PUB-060731-9
- Date
- 2006
- Source
- The Journal of biological chemistry 281(37): 27454-27460 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Humans
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Neovascularization, Pathologic
- Neovascularization, Physiologic
- Phylogeny
- Ribonuclease, Pancreatic/chemistry*
- Ribonuclease, Pancreatic/metabolism
- Ribonucleases/chemistry*
- Ribonucleases/metabolism
- Sequence Homology, Amino Acid
- Zebrafish
- PubMed
- 16861230 Full text @ J. Biol. Chem.
Citation
Pizzo, E., Buonanno, P., Di Maro, A., Ponticelli, S., De Falco, S., Quarto, N., Cubellis, M.V., and D'Alessio, G. (2006) Ribonucleases and angiogenins from fish. The Journal of biological chemistry. 281(37):27454-27460.
Abstract
For the first time fish RNases have been isolated and characterized. Their functional and structural properties indicate that they belong to the "RNase A superfamily", or "tetrapod RNase superfamily", now more appropriately described as the "Vertebrate RNase Superfamily". Our findings suggest how many previous, repeated efforts to isolate RNases from fish tissues have met with no success: fish RNases have a very low ribonucleolytic activity, and their genes have a low sequence identity with those of mammalian RNases. The investigated RNases are from the bony fish Danio rerio, or zebrafish. Their cDNAs have been cloned, expressed and the three recombinant proteins have been purified to homogeneity. The main findings of their characterization has revealed: That they have indeed a very low RNA degrading activity, when compared to that of RNase A, the superfamily prototype, but comparable to that of mammalian angiogenins; that two of them have angiogenic activity, which is inhibited by the cytosolic RNase inhibitor. These data, and a phylogenetic analysis indicate that angiogenic fish RNases are the earliest diverging members of the vertebrate superfamily, and suggest that ribonucleases with angiogenic activity were the ancestors of all ribonucleases in the superfamily. They later evolved both into mammalian angiogenins, and through a successful phylogenesis into RNases endowed with digestive features or with diverse bioactions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping