ZFIN ID: ZDB-PUB-060710-10
Identification and analysis of putative regulatory sequences for the MYF5/MYF6 locus in different vertebrate species
Maak, S., Neumann, K., and Swalve, H.H.
Date: 2006
Source: Gene 379: 141-147 (Journal)
Registered Authors:
Keywords: Comparative genomics, Gene regulation, Enhancer, Porcine SNPs
MeSH Terms:
  • Animals
  • Cattle
  • Chickens/genetics
  • Conserved Sequence
  • Dogs
  • Humans
  • Mice
  • Models, Genetic
  • Muscle Development
  • Muscle, Skeletal
  • Myogenic Regulatory Factor 5/genetics*
  • Myogenic Regulatory Factor 5/metabolism
  • Myogenic Regulatory Factors/genetics*
  • Polymorphism, Single Nucleotide
  • Regulatory Elements, Transcriptional*
  • Sequence Analysis
  • Species Specificity
  • Swine/genetics
  • Zebrafish/genetics
PubMed: 16820272 Full text @ Gene
The myogenic factors (MYF) 5 and 6 are integral to the initiation and development of skeletal muscle and to the maintenance of its phenotype. Thus, they are candidate genes for growth- and meat quality-related traits. We performed a comparative sequence analysis of the MYF5/MYF6 locus in swine, cattle, dog, chicken and zebrafish on the basis of structural and functional information from human and mouse. Beside the characterization of upstream regulatory elements recently identified in mice, we demonstrate the existence of further highly conserved elements (E1 to E4) which may play a role in the regulation of MYF5 and MYF6 expression. Comparative sequence analysis of putative regulatory sequences in swine revealed a total of 21 single nucleotide polymorphisms (SNP) including 1 and 6 SNPs new for the promoters of MYF5 and MYF6, respectively. The conserved organization of the locus in vertebrates indicates a common basic mechanism of muscle development. However, the existence of numerous regulatory elements at large distances to MYF5 and MYF6 points to a very complex pattern of the gene regulation with significant differences between species.