PUBLICATION
Efficient Transfection Strategy for the Spatiotemporal Control of Gene Expression in Zebrafish
- Authors
- Ando, H., and Okamoto, H.
- ID
- ZDB-PUB-060419-5
- Date
- 2006
- Source
- Marine biotechnology (New York, N.Y.) 8(3): 295-303 (Journal)
- Registered Authors
- Ando, Hideki
- Keywords
- Gene expression, lipofection, morpholino oligonucleotides, transfection, zebrafish
- MeSH Terms
-
- Animals
- Brain/embryology
- Brain/metabolism
- DNA/genetics
- DNA/metabolism
- Eye/embryology
- Eye/metabolism
- Gene Expression Regulation, Developmental*
- Larva/genetics
- Larva/metabolism
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Transfection/methods*
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 16614871 Full text @ Mar. Biotechnol.
Citation
Ando, H., and Okamoto, H. (2006) Efficient Transfection Strategy for the Spatiotemporal Control of Gene Expression in Zebrafish. Marine biotechnology (New York, N.Y.). 8(3):295-303.
Abstract
Functional analyses of gene function by knockdown and expression approaches strongly enhance the genetic study of development. In vivo application of the introduction of inhibitors of gene expression, mRNA, and expression constructs in the target region make it possible to perform region- and stage-specific regulation of gene function in a simple manner. As a basic tool for the conditional regulation of gene expression in target tissue, we present methods for the efficient introduction of antisense morpholino oligonucleotide (MO), mRNA, and expression plasmid constructs into early and later stage zebrafish embryo and larva. Lipofection of a neuron-specific expression construct plasmid encoding green fluorescent protein (GFP) into optic vesicle resulted in clear GFP expression in the retinotectal pathway in hatched larva. Co-lipofection of MO and GFP mRNA to the presumptive head region resulted in brain-specific knockdown of the gene in mid-stage embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping