PUBLICATION
Conservation of RET Regulatory Function from Human to Zebrafish Without Sequence Similarity
- Authors
- Fisher, S., Grice, E.A., Vinton, R.M., Bessling, S.L., and McCallion, A.S.
- ID
- ZDB-PUB-060327-5
- Date
- 2006
- Source
- Science (New York, N.Y.) 312(5771): 276-279 (Journal)
- Registered Authors
- Fisher, Shannon
- Keywords
- none
- MeSH Terms
-
- Animals
- Conserved Sequence*
- Enhancer Elements, Genetic*
- Gene Expression Regulation*
- Humans
- Models, Genetic
- Neurons/metabolism
- Proto-Oncogene Proteins c-ret/genetics*
- Regulatory Sequences, Nucleic Acid*
- Sequence Analysis, DNA
- Takifugu/genetics
- Transgenes
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 16556802 Full text @ Science
Citation
Fisher, S., Grice, E.A., Vinton, R.M., Bessling, S.L., and McCallion, A.S. (2006) Conservation of RET Regulatory Function from Human to Zebrafish Without Sequence Similarity. Science (New York, N.Y.). 312(5771):276-279.
Abstract
Evolutionary sequence conservation is an accepted criterion to identify noncoding regulatory sequences. We have used a transposon-based transgenic assay in zebrafish to evaluate noncoding sequences at the zebrafish ret locus, conserved among teleosts, and at the human RET locus, conserved among mammals. Most teleost sequences directed ret-specific reporter gene expression, with many displaying overlapping regulatory control. The majority of human RET noncoding sequences also directed ret-specific expression in zebrafish. Thus, vast amounts of functional sequence information may exist that would not be detected by sequence similarity approaches.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping